This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-08-26    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to ATPase activity	IDA: Inferred from Direct Assay Assigned on 2008-09-05	Lau WC, et al.  (2008) Cryo-EM structure of the yeast ATP synthase. J Mol Biol 382(5):1256-64	SGD
contributes_to proton-transporting ATP synthase activity, rotational mechanism	ISS: Inferred from Sequence or structural Similarity Assigned on 2006-06-29 IMP: Inferred from Mutant Phenotype Assigned on 2006-06-29	Saltzgaber-Muller J, et al.  (1983) Nuclear genes coding the yeast mitochondrial adenosine triphosphatase complex. Isolation of ATP2 coding the F1-ATPase beta subunit. J Biol Chem 258(19):11465-70	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-08-26	Ichikawa N and Mizuno M  (2004) Functional expression of hexahistidine-tagged beta-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography. Protein Expr Purif 37(1):97-101	SGD
proton-transporting ATPase activity, rotational mechanism	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-26	Mueller DM  (1989) Site directed mutagenesis of the beta-subunit of the yeast mitochondrial ATPase. Biochem Biophys Res Commun 164(1):381-6	SGD
contributes_to proton-transporting ATPase activity, rotational mechanism	IDA: Inferred from Direct Assay Assigned on 2011-01-21	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP synthesis coupled proton transport	ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Saltzgaber-Muller J, et al.  (1983) Nuclear genes coding the yeast mitochondrial adenosine triphosphatase complex. Isolation of ATP2 coding the F1-ATPase beta subunit. J Biol Chem 258(19):11465-70	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-26	Mueller DM  (1989) Site directed mutagenesis of the beta-subunit of the yeast mitochondrial ATPase. Biochem Biophys Res Commun 164(1):381-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-01-21	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial proton-transporting ATP synthase, catalytic core	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Saltzgaber-Muller J, et al.  (1983) Nuclear genes coding the yeast mitochondrial adenosine triphosphatase complex. Isolation of ATP2 coding the F1-ATPase beta subunit. J Biol Chem 258(19):11465-70	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-05-07	Kabaleeswaran V, et al.  (2006) Novel features of the rotary catalytic mechanism revealed in the structure of yeast F1 ATPase. EMBO J 25(22):5433-42	SGD
mitochondrion	IPI: Inferred from Physical Interaction with SGD:ENO2, SGD:ENO1 Assigned on 2007-03-05	Brandina I, et al.  (2006) Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta 1757(9-10):1217-1228	SGD
proton-transporting ATP synthase complex, catalytic core F(1)	IDA: Inferred from Direct Assay Assigned on 2008-08-26	Ichikawa N and Mizuno M  (2004) Functional expression of hexahistidine-tagged beta-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography. Protein Expr Purif 37(1):97-101	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR020003, EBI:IPR000194 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	IEA: Inferred from Electronic Annotation with EBI:IPR000793 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleoside-triphosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003593 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR003593 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
proton-transporting ATP synthase activity, rotational mechanism	IEA: Inferred from Electronic Annotation with EBI:IPR005722 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:KW-0066 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ATP hydrolysis coupled proton transport	IEA: Inferred from Electronic Annotation with EBI:IPR000793 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATP metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR004100 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATP synthesis coupled proton transport	IEA: Inferred from Electronic Annotation with EBI:IPR005722 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ion transport	IEA: Inferred from Electronic Annotation with EBI:KW-0406 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
proton transport	IEA: Inferred from Electronic Annotation with EBI:IPR004100 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0375 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrial inner membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0168 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0999 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:SL-0173 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
proton-transporting ATP synthase complex, catalytic core F(1)	IEA: Inferred from Electronic Annotation with EBI:IPR005722 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0139 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
proton-transporting two-sector ATPase complex, catalytic domain	IEA: Inferred from Electronic Annotation with EBI:IPR000793 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).