This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-07-19    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
signal transducer activity	IMP: Inferred from Mutant Phenotype Assigned on 2006-07-19	Nomoto S, et al.  (1990) Regulation of the yeast pheromone response pathway by G protein subunits. EMBO J 9(3):691-6	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
heterotrimeric G-protein complex cycle	IMP: Inferred from Mutant Phenotype Assigned on 2006-07-19	Nomoto S, et al.  (1990) Regulation of the yeast pheromone response pathway by G protein subunits. EMBO J 9(3):691-6	SGD
pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP: Inferred from Mutant Phenotype Assigned on 2002-12-06	Whiteway M, et al.  (1988) Function of the STE4 and STE18 genes in mating pheromone signal transduction in Saccharomyces cerevisiae. Cold Spring Harb Symp Quant Biol 53 Pt 2:585-90	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Hirschman JE, et al.  (1997) The G beta gamma complex of the yeast pheromone response pathway. Subcellular fractionation and protein-protein interactions. J Biol Chem 272(1):240-8	SGD
heterotrimeric G-protein complex	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Hirschman JE, et al.  (1997) The G beta gamma complex of the yeast pheromone response pathway. Subcellular fractionation and protein-protein interactions. J Biol Chem 272(1):240-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2006-07-19	Cole GM, et al.  (1990) Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway. Mol Cell Biol 10(2):510-7	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Hirschman JE, et al.  (1997) The G beta gamma complex of the yeast pheromone response pathway. Subcellular fractionation and protein-protein interactions. J Biol Chem 272(1):240-8	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for STE18

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
signal transducer activity	IEA: Inferred from Electronic Annotation with EBI:IPR015898 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0807 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
G-protein coupled receptor signaling pathway	IEA: Inferred from Electronic Annotation with EBI:IPR015898 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
response to pheromone	IEA: Inferred from Electronic Annotation with EBI:KW-0589 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
signal transduction	IEA: Inferred from Electronic Annotation with EBI:KW-0807 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
heterotrimeric G-protein complex	IEA: Inferred from Electronic Annotation with EBI:IPR015898 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0162 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).