This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-11-17    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2008-07-20	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial respiratory chain complex IV assembly	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	McEwen JE, et al.  (1993) Sequence and chromosomal localization of two PET genes required for cytochrome c oxidase assembly in Saccharomyces cerevisiae. Curr Genet 23(1):9-14	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-07-20	Khalimonchuk O, et al.  (2008) Pet191 is a cytochrome c oxidase assembly factor in Saccharomyces cerevisiae. Eukaryot Cell 7(8):1427-31	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to mitochondrial inner membrane	IDA: Inferred from Direct Assay Assigned on 2008-07-20	Khalimonchuk O, et al.  (2008) Pet191 is a cytochrome c oxidase assembly factor in Saccharomyces cerevisiae. Eukaryot Cell 7(8):1427-31	SGD
mitochondrial intermembrane space	IDA: Inferred from Direct Assay Assigned on 2012-10-29	Voegtle FN, et al.  (2012) Intermembrane space proteome of yeast mitochondria. Mol Cell Proteomics 11(12):1840-52	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:SL-0173 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).