This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-05-09    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
SNARE binding	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-11 IPI: Inferred from Physical Interaction Assigned on 2011-02-11	Galan JM, et al.  (2001) Skp1p and the F-box protein Rcy1p form a non-SCF complex involved in recycling of the SNARE Snc1p in yeast. Mol Cell Biol 21(9):3105-17	SGD
	IPI: Inferred from Physical Interaction Assigned on 2011-02-11	Chen SH, et al.  (2005) Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell 16(1):178-92	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
early endosome to Golgi transport	IMP: Inferred from Mutant Phenotype Assigned on 2010-12-10	Chen SH, et al.  (2005) Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell 16(1):178-92	SGD
endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2002-10-04	Wiederkehr A, et al.  (2001) Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid-phase endocytosis. Yeast 18(8):759-73	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-10-04	Wiederkehr A, et al.  (2000) The F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae. J Cell Biol 149(2):397-410	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endosome	IDA: Inferred from Direct Assay Assigned on 2005-07-11	Chen SH, et al.  (2005) Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell 16(1):178-92	SGD
Golgi apparatus	IDA: Inferred from Direct Assay Assigned on 2005-07-11	Chen SH, et al.  (2005) Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell 16(1):178-92	SGD
site of polarized growth	IDA: Inferred from Direct Assay Assigned on 2003-05-09	Galan JM, et al.  (2001) Skp1p and the F-box protein Rcy1p form a non-SCF complex involved in recycling of the SNARE Snc1p in yeast. Mol Cell Biol 21(9):3105-17	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for RCY1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
exocytosis	IEA: Inferred from Electronic Annotation with EBI:IPR009976 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
vesicle docking	IEA: Inferred from Electronic Annotation with EBI:IPR009976 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cell tip	IEA: Inferred from Electronic Annotation with EBI:SL-0456 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
cellular bud neck	IEA: Inferred from Electronic Annotation with EBI:SL-0029 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:IPR009976 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).