This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-03-31    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein tyrosine kinase activity	IDA: Inferred from Direct Assay Assigned on 2010-11-19	Booher RN, et al.  (1993) Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J 12(9):3417-26	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell shape checkpoint	IDA: Inferred from Direct Assay Assigned on 2002-01-28	Sia RA, et al.  (1996) Cdc28 tyrosine phosphorylation and the morphogenesis checkpoint in budding yeast. Mol Biol Cell 7(11):1657-66	SGD
	IEP: Inferred from Expression Pattern Assigned on 2006-03-31 IMP: Inferred from Mutant Phenotype Assigned on 2006-03-31	McMillan JN, et al.  (1998) A morphogenesis checkpoint monitors the actin cytoskeleton in yeast. J Cell Biol 142(6):1487-99	SGD
NOT G2 cell size control checkpoint	IEP: Inferred from Expression Pattern Assigned on 2006-03-27 IMP: Inferred from Mutant Phenotype Assigned on 2006-03-27	McNulty JJ and Lew DJ  (2005) Swe1p responds to cytoskeletal perturbation, not bud size, in S. cerevisiae. Curr Biol 15(24):2190-8	SGD
G2/M transition of mitotic cell cycle	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-03-20 IMP: Inferred from Mutant Phenotype Assigned on 2002-03-20 IDA: Inferred from Direct Assay Assigned on 2002-03-20	Booher RN, et al.  (1993) Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J 12(9):3417-26	SGD
negative regulation of spindle pole body separation	IGI: Inferred from Genetic Interaction with SGD:CDC28 Assigned on 2009-02-13 IMP: Inferred from Mutant Phenotype Assigned on 2009-02-13	Lim HH, et al.  (1996) Spindle pole body separation in Saccharomyces cerevisiae requires dephosphorylation of the tyrosine 19 residue of Cdc28. Mol Cell Biol 16(11):6385-97	SGD
re-entry into mitotic cell cycle	IGI: Inferred from Genetic Interaction with SGD:RIX1, SGD:SDA1, SGD:TIF6 Assigned on 2008-07-11	Saracino F, et al.  (2004) The yeast kinase Swe1 is required for proper entry into cell cycle after arrest due to ribosome biogenesis and protein synthesis defects. Cell Cycle 3(5):648-54	SGD
regulation of cyclin-dependent protein serine/threonine kinase activity	IDA: Inferred from Direct Assay Assigned on 2002-01-28	Booher RN, et al.  (1993) Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J 12(9):3417-26	SGD
regulation of meiosis	IGI: Inferred from Genetic Interaction Assigned on 2002-06-18 IEP: Inferred from Expression Pattern Assigned on 2002-06-18 IMP: Inferred from Mutant Phenotype Assigned on 2002-06-18	Leu JY and Roeder GS  (1999) The pachytene checkpoint in S. cerevisiae depends on Swe1-mediated phosphorylation of the cyclin-dependent kinase Cdc28. Mol Cell 4(5):805-14	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2002-01-28	Longtine MS, et al.  (2000) Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol Cell Biol 20(11):4049-61	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-10-08	Sundin BA, et al.  (2004) Localization of proteins that are coordinately expressed with Cln2 during the cell cycle. Yeast 21(9):793-800	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-01-28	Longtine MS, et al.  (2000) Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol Cell Biol 20(11):4049-61	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-10-08	Sundin BA, et al.  (2004) Localization of proteins that are coordinately expressed with Cln2 during the cell cycle. Yeast 21(9):793-800	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function | Biological Process

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein kinase activity	IDA: Inferred from Direct Assay Assigned on 2012-05-04	Ptacek J, et al.  (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84	SGD

High-throughput Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein phosphorylation	IDA: Inferred from Direct Assay Assigned on 2012-05-04	Ptacek J, et al.  (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84	SGD
regulation of cell size	IMP: Inferred from Mutant Phenotype Assigned on 2006-07-14	Jorgensen P, et al.  (2002) Systematic identification of pathways that couple cell growth and division in yeast. Science 297(5580):395-400	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR017441, EBI:IPR000719 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
kinase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000719 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein serine/threonine kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR008271 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0723 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein tyrosine kinase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0829 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring phosphorus-containing groups	IEA: Inferred from Electronic Annotation with EBI:IPR011009 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
meiosis	IEA: Inferred from Electronic Annotation with EBI:KW-0469 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitosis	IEA: Inferred from Electronic Annotation with EBI:KW-0498 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphorylation	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein phosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR008271 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IEA: Inferred from Electronic Annotation with EBI:SL-0029 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).