This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-05-30    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphatidylinositol-3-phosphate binding	IDA: Inferred from Direct Assay Assigned on 2008-05-30	Wurmser AE and Emr SD  (2002) Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy. J Cell Biol 158(4):761-72	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagic vacuole assembly	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-30	He C, et al.  (2007) Atg27 is required for autophagy-dependent cycling of Atg9. Mol Biol Cell 18(2):581-93	SGD
CVT pathway	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-30	He C, et al.  (2007) Atg27 is required for autophagy-dependent cycling of Atg9. Mol Biol Cell 18(2):581-93	SGD
peroxisome degradation	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-30	He C, et al.  (2007) Atg27 is required for autophagy-dependent cycling of Atg9. Mol Biol Cell 18(2):581-93	SGD
protein localization to pre-autophagosomal structure	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-05	He C, et al.  (2007) Atg27 is required for autophagy-dependent cycling of Atg9. Mol Biol Cell 18(2):581-93	SGD
vesicle organization	IMP: Inferred from Mutant Phenotype Assigned on 2003-02-25	Wurmser AE and Emr SD  (2002) Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy. J Cell Biol 158(4):761-72	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
membrane	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-02-25 IDA: Inferred from Direct Assay Assigned on 2003-02-25	Wurmser AE and Emr SD  (2002) Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy. J Cell Biol 158(4):761-72	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2007-03-08	He C, et al.  (2007) Atg27 is required for autophagy-dependent cycling of Atg9. Mol Biol Cell 18(2):581-93	SGD
pre-autophagosomal structure	IDA: Inferred from Direct Assay Assigned on 2007-03-08	He C, et al.  (2007) Atg27 is required for autophagy-dependent cycling of Atg9. Mol Biol Cell 18(2):581-93	SGD
trans-Golgi network	IDA: Inferred from Direct Assay Assigned on 2008-05-30	Inadome H, et al.  (2005) Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments. Mol Cell Biol 25(17):7696-710	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for ATG27

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagy	IEA: Inferred from Electronic Annotation with EBI:KW-0072 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasmic vesicle	IEA: Inferred from Electronic Annotation with EBI:KW-0968 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoplasmic vesicle membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0089 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
Golgi apparatus	IEA: Inferred from Electronic Annotation with EBI:KW-0333 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
Golgi membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0134 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrial membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0171 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).