This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-09-18    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
double-stranded DNA binding	IDA: Inferred from Direct Assay Assigned on 2007-09-18	Singh KK and Samson L  (1995) Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes. Proc Natl Acad Sci U S A 92(11):4907-11	SGD
sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2007-09-18	Singh KK and Samson L  (1995) Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes. Proc Natl Acad Sci U S A 92(11):4907-11	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA repair	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-18	Maniar HS, et al.  (1997) Roles of replication protein-A subunits 2 and 3 in DNA replication fork movement in Saccharomyces cerevisiae. Genetics 145(4):891-902	SGD
DNA replication	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-18	Maniar HS, et al.  (1997) Roles of replication protein-A subunits 2 and 3 in DNA replication fork movement in Saccharomyces cerevisiae. Genetics 145(4):891-902	SGD
DNA unwinding involved in replication	IDA: Inferred from Direct Assay Assigned on 2007-09-18	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD
double-strand break repair via homologous recombination	IGI: Inferred from Genetic Interaction with SGD:SPO11 Assigned on 2006-12-22	Gasior SL, et al.  (1998) Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes. Genes Dev 12(14):2208-21	SGD
establishment of protein localization	IPI: Inferred from Physical Interaction with SGD:RFA2 Assigned on 2007-09-18	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD
heteroduplex formation	IDA: Inferred from Direct Assay Assigned on 2007-09-18	Van Komen S, et al.  (2002) Functional cross-talk among Rad51, Rad54, and replication protein A in heteroduplex DNA joint formation. J Biol Chem 277(46):43578-87	SGD
mitotic recombination	IPI: Inferred from Physical Interaction with SGD:RFA1 Assigned on 2007-09-18	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD
nucleotide-excision repair	IDA: Inferred from Direct Assay Assigned on 2007-09-18	He Z, et al.  (1996) Assessing the requirements for nucleotide excision repair proteins of Saccharomyces cerevisiae in an in vitro system. J Biol Chem 271(45):28243-9	SGD
protein ubiquitination	IPI: Inferred from Physical Interaction with SGD:RFA1 Assigned on 2008-09-09	Davies AA, et al.  (2008) Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein a. Mol Cell 29(5):625-36	SGD
reciprocal meiotic recombination	IPI: Inferred from Physical Interaction with SGD:RFA1 Assigned on 2007-09-18	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD
telomere maintenance via recombination	IPI: Inferred from Physical Interaction with SGD:RFA1 Assigned on 2007-09-18	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD
telomere maintenance via telomerase	IPI: Inferred from Physical Interaction with SGD:RFA2 Assigned on 2007-09-18	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome, telomeric region	IMP: Inferred from Mutant Phenotype Assigned on 2004-03-27	Schramke V, et al.  (2004) RPA regulates telomerase action by providing Est1p access to chromosome ends. Nat Genet 36(1):46-54	SGD
condensed nuclear chromosome	IDA: Inferred from Direct Assay Assigned on 2007-09-18	Gasior SL, et al.  (1998) Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes. Genes Dev 12(14):2208-21	SGD
DNA replication factor A complex	IDA: Inferred from Direct Assay Assigned on 2007-09-18	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for RFA3

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication	IEA: Inferred from Electronic Annotation with EBI:KW-0235 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).