This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-07-06    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
histone methyltransferase activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2005-05-25	Boa S, et al.  (2003) Saccharomyces cerevisiae Set1p is a methyltransferase specific for lysine 4 of histone H3 and is required for efficient gene expression. Yeast 20(9):827-35	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2005-05-25	Strahl BD, et al.  (2002) Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol Cell Biol 22(5):1298-306	SGD
histone methyltransferase activity (H3-K36 specific)	IMP: Inferred from Mutant Phenotype Assigned on 2005-05-25 IDA: Inferred from Direct Assay Assigned on 2005-05-25	Strahl BD, et al.  (2002) Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol Cell Biol 22(5):1298-306	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ascospore formation	IMP: Inferred from Mutant Phenotype Assigned on 2006-12-15	Morohashi N, et al.  (2005) Effect of histone methyltransferase gene mutations on sporulation in S. cerevisiae. Nucleic Acids Symp Ser (Oxf)(49):325-6	SGD
DNA-dependent transcription, elongation	IDA: Inferred from Direct Assay Assigned on 2003-07-06 IPI: Inferred from Physical Interaction Assigned on 2003-07-06	Schaft D, et al.  (2003) The histone 3 lysine 36 methyltransferase, SET2, is involved in transcriptional elongation. Nucleic Acids Res 31(10):2475-82	SGD
histone deacetylation	IMP: Inferred from Mutant Phenotype Assigned on 2011-07-20	Carrozza MJ, et al.  (2005) Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription. Cell 123(4):581-92	SGD
histone methylation	IMP: Inferred from Mutant Phenotype Assigned on 2002-05-22 IDA: Inferred from Direct Assay Assigned on 2002-05-22	Strahl BD, et al.  (2002) Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol Cell Biol 22(5):1298-306	SGD
negative regulation of antisense RNA transcription	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-11	Churchman LS and Weissman JS  (2011) Nascent transcript sequencing visualizes transcription at nucleotide resolution. Nature 469(7330):368-73	SGD
negative regulation of histone H3-K14 acetylation	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-11	Quan TK and Hartzog GA  (2010) Histone H3K4 and K36 Methylation, Chd1 and Rpd3S Oppose the Functions of Saccharomyces cerevisiae Spt4-Spt5 in Transcription. Genetics 184(2):321-34	SGD
negative regulation of histone H3-K9 acetylation	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-11	Quan TK and Hartzog GA  (2010) Histone H3K4 and K36 Methylation, Chd1 and Rpd3S Oppose the Functions of Saccharomyces cerevisiae Spt4-Spt5 in Transcription. Genetics 184(2):321-34	SGD
negative regulation of reciprocal meiotic recombination	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-12	Merker JD, et al.  (2008) The histone methylase Set2p and the histone deacetylase Rpd3p repress meiotic recombination at the HIS4 meiotic recombination hotspot in Saccharomyces cerevisiae. DNA Repair (Amst) 7(8):1298-308	SGD
positive regulation of histone acetylation	IGI: Inferred from Genetic Interaction with SGD:SET1 Assigned on 2009-10-29	Ginsburg DS, et al.  (2009) NuA4 Lysine Acetyltransferase Esa1 Is Targeted to Coding Regions and Stimulates Transcription Elongation with Gcn5. Mol Cell Biol 29(24):6473-87	SGD
regulation of DNA-dependent DNA replication initiation	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-13	Knott SR, et al.  (2009) Genome-wide replication profiles indicate an expansive role for Rpd3L in regulating replication initiation timing or efficiency, and reveal genomic loci of Rpd3 function in Saccharomyces cerevisiae. Genes Dev 23(9):1077-90	SGD
regulation of transcription, DNA-dependent	IMP: Inferred from Mutant Phenotype Assigned on 2002-05-22 IDA: Inferred from Direct Assay Assigned on 2002-05-22	Strahl BD, et al.  (2002) Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol Cell Biol 22(5):1298-306	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IPI: Inferred from Physical Interaction Assigned on 2002-05-22	Strahl BD, et al.  (2002) Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol Cell Biol 22(5):1298-306	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SET2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
histone methyltransferase activity (H3-K36 specific)	IEA: Inferred from Electronic Annotation with EBI:IPR025788 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
histone-lysine N-methyltransferase activity	IEA: Inferred from Electronic Annotation with EBI:IPR013257, EBI:IPR006560 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:2.1.1.43 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
methyltransferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0489 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-dependent transcription, elongation	IEA: Inferred from Electronic Annotation with EBI:IPR025788 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
histone H3-K36 methylation	IEA: Inferred from Electronic Annotation with EBI:IPR025788 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
histone lysine methylation	IEA: Inferred from Electronic Annotation with EBI:IPR013257 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
methylation	IEA: Inferred from Electronic Annotation with EBI:KW-0489 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:IPR013257 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome	IEA: Inferred from Electronic Annotation with EBI:IPR013257 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0468 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0158 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR006560 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).