This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-10-04    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
histone binding	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-15	Mousson F, et al.  (2005) Structural basis for the interaction of Asf1 with histone H3 and its functional implications. Proc Natl Acad Sci U S A 102(17):5975-80	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin silencing at silent mating-type cassette	IGI: Inferred from Genetic Interaction with SGD:RLF2 Assigned on 2006-09-15	Mousson F, et al.  (2005) Structural basis for the interaction of Asf1 with histone H3 and its functional implications. Proc Natl Acad Sci U S A 102(17):5975-80	SGD
chromatin silencing at telomere	IGI: Inferred from Genetic Interaction with SGD:RLF2 Assigned on 2006-09-15	Mousson F, et al.  (2005) Structural basis for the interaction of Asf1 with histone H3 and its functional implications. Proc Natl Acad Sci U S A 102(17):5975-80	SGD
DNA replication-dependent nucleosome assembly	IDA: Inferred from Direct Assay Assigned on 2006-09-21	Sharp JA, et al.  (2001) Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr Biol 11(7):463-73	SGD
DNA replication-independent nucleosome assembly	IDA: Inferred from Direct Assay Assigned on 2006-09-21	Green EM, et al.  (2005) Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol 15(22):2044-9	SGD
histone acetylation	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-21	Recht J, et al.  (2006) Histone chaperone Asf1 is required for histone H3 lysine 56 acetylation, a modification associated with S phase in mitosis and meiosis. Proc Natl Acad Sci U S A 103(18):6988-93	SGD
histone exchange	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-20	Rufiange A, et al.  (2007) Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3 K56 acetylation and Asf1. Mol Cell 27(3):393-405	SGD
nucleosome disassembly	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-21	Schwabish MA and Struhl K  (2006) Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II. Mol Cell 22(3):415-22	SGD
positive regulation of histone acetylation	IGI: Inferred from Genetic Interaction with SGD:GCN5 Assigned on 2008-08-27 IMP: Inferred from Mutant Phenotype Assigned on 2008-08-27 IDA: Inferred from Direct Assay Assigned on 2008-08-27	Fillingham J, et al.  (2008) Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109. Mol Cell Biol 28(13):4342-53	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-27 IDA: Inferred from Direct Assay Assigned on 2008-08-27	Driscoll R, et al.  (2007) Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56. Science 315(5812):649-52	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-27 IPI: Inferred from Physical Interaction with SGD:RTT109, SGD:VPS75, SGD:HHF2, SGD:HHF1, SGD:HHT2, SGD:HHT1 Assigned on 2008-08-27	Han J, et al.  (2007) Acetylation of Lysine 56 of Histone H3 Catalyzed by RTT109 and Regulated by ASF1 Is Required for Replisome Integrity. J Biol Chem 282(39):28587-96	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-27	Rufiange A, et al.  (2007) Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3 K56 acetylation and Asf1. Mol Cell 27(3):393-405	SGD
positive regulation of transcription elongation from RNA polymerase II promoter	IDA: Inferred from Direct Assay Assigned on 2013-03-07	Kuryan BG, et al.  (2012) Histone density is maintained during transcription mediated by the chromatin remodeler RSC and histone chaperone NAP1 in vitro. Proc Natl Acad Sci U S A 109(6):1931-6	SGD
regulation of transcription from RNA polymerase II promoter in response to stress	IMP: Inferred from Mutant Phenotype Assigned on 2009-07-27	Klopf E, et al.  (2009) Cooperation between the INO80 complex and histone chaperones determines adaptation of stress gene transcription in the yeast Saccharomyces cerevisiae. Mol Cell Biol 29(18):4994-5007	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2013-06-06	Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2013-06-06	Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30	SGD
	IDA: Inferred from Direct Assay Assigned on 2013-02-15	Sutton A, et al.  (2001) Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription. Genetics 158(2):587-96	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for ASF1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin assembly or disassembly	IEA: Inferred from Electronic Annotation with EBI:IPR006818 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
chromatin modification	IEA: Inferred from Electronic Annotation with EBI:KW-0156 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR006818 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).