This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-11-07    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein binding, bridging	IPI: Inferred from Physical Interaction with SGD:RSP5, SGD:DIP5 Assigned on 2011-02-18	Hatakeyama R, et al.  (2010) Endocytosis of the Aspartic Acid/Glutamic Acid Transporter Dip5 Is Triggered by Substrate-Dependent Recruitment of the Rsp5 Ubiquitin Ligase via the Arrestin-Like Protein Aly2. Mol Cell Biol 30(24):5598-607	SGD
ubiquitin protein ligase binding	IPI: Inferred from Physical Interaction with SGD:RSP5 Assigned on 2008-11-07	Lin CH, et al.  (2008) Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell 135(4):714-25	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
positive regulation of ubiquitin-dependent endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-25	Hatakeyama R, et al.  (2010) Endocytosis of the Aspartic Acid/Glutamic Acid Transporter Dip5 Is Triggered by Substrate-Dependent Recruitment of the Rsp5 Ubiquitin Ligase via the Arrestin-Like Protein Aly2. Mol Cell Biol 30(24):5598-607	SGD
regulation of intracellular transport	IMP: Inferred from Mutant Phenotype Assigned on 2011-03-14	O'Donnell AF, et al.  (2010) {alpha}-Arrestins Aly1 and Aly2 Regulate Intracellular Trafficking in Response to Nutrient Signaling. Mol Biol Cell 21(20):3552-66	SGD
ubiquitin-dependent endocytosis	ISA: Inferred from Sequence Alignment with SGD:LDB19 Assigned on 2009-10-05	Lin CH, et al.  (2008) Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell 135(4):714-25	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
early endosome	IDA: Inferred from Direct Assay Assigned on 2011-03-14	O'Donnell AF, et al.  (2010) {alpha}-Arrestins Aly1 and Aly2 Regulate Intracellular Trafficking in Response to Nutrient Signaling. Mol Biol Cell 21(20):3552-66	SGD
late endosome	IDA: Inferred from Direct Assay Assigned on 2011-03-14	O'Donnell AF, et al.  (2010) {alpha}-Arrestins Aly1 and Aly2 Regulate Intracellular Trafficking in Response to Nutrient Signaling. Mol Biol Cell 21(20):3552-66	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).