This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-07-15    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
polyphosphate kinase activity	IDA: Inferred from Direct Assay Assigned on 2009-09-18	Hothorn M, et al.  (2009) Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase. Science 324(5926):513-6	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
microautophagy	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-22 IDA: Inferred from Direct Assay Assigned on 2007-08-23	Uttenweiler A, et al.  (2007) The vacuolar transporter chaperone (VTC) complex is required for microautophagy. Mol Biol Cell 18(1):166-75	SGD
polyphosphate metabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-17	Hothorn M, et al.  (2009) Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase. Science 324(5926):513-6	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-18	Ogawa N, et al.  (2000) New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis. Mol Biol Cell 11(12):4309-21	SGD
vacuolar transport	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-23 IDA: Inferred from Direct Assay Assigned on 2007-08-23	Uttenweiler A, et al.  (2007) The vacuolar transporter chaperone (VTC) complex is required for microautophagy. Mol Biol Cell 18(1):166-75	SGD
vacuole fusion, non-autophagic	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-15	Muller O, et al.  (2002) The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation. EMBO J 21(3):259-69	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum	IDA: Inferred from Direct Assay Assigned on 2007-08-23	Uttenweiler A, et al.  (2007) The vacuolar transporter chaperone (VTC) complex is required for microautophagy. Mol Biol Cell 18(1):166-75	SGD
intrinsic to vacuolar membrane	IDA: Inferred from Direct Assay Assigned on 2005-05-05	Muller O, et al.  (2003) Role of the Vtc proteins in V-ATPase stability and membrane trafficking. J Cell Sci 116(Pt 6):1107-15	SGD
vacuolar transporter chaperone complex	IPI: Inferred from Physical Interaction with SGD:VTC1 Assigned on 2007-08-22	Muller O, et al.  (2002) The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation. EMBO J 21(3):259-69	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuolar membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0271 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuole	IEA: Inferred from Electronic Annotation with EBI:KW-0926 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).