This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-04-07    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
alpha-glucoside:hydrogen symporter activity	IDA: Inferred from Direct Assay Assigned on 2003-04-29	Stambuk BU and de Araujo PS  (2001) Kinetics of active alpha-glucoside transport in Saccharomyces cerevisiae. FEMS Yeast Res 1(1):73-8	SGD
maltose:hydrogen symporter activity	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-07	Han EK, et al.  (1995) Characterization of AGT1 encoding a general alpha-glucoside transporter from Saccharomyces. Mol Microbiol 17(6):1093-107	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-05-31	Cheng Q and Michels CA  (1991) MAL11 and MAL61 encode the inducible high-affinity maltose transporter of Saccharomyces cerevisiae. J Bacteriol 173(5):1817-20	SGD
trehalose transmembrane transporter activity	IDA: Inferred from Direct Assay Assigned on 2003-04-29	Stambuk BU and de Araujo PS  (2001) Kinetics of active alpha-glucoside transport in Saccharomyces cerevisiae. FEMS Yeast Res 1(1):73-8	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
disaccharide catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-28	Teste MA, et al.  (2010) Characterization of a New Multigene Family Encoding Isomaltases in the Yeast Saccharomyces cerevisiae, the IMA Family. J Biol Chem 285(35):26815-24	SGD
maltose transport	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-07	Han EK, et al.  (1995) Characterization of AGT1 encoding a general alpha-glucoside transporter from Saccharomyces. Mol Microbiol 17(6):1093-107	SGD
	IGI: Inferred from Genetic Interaction with SGD:MAL61 Assigned on 2008-04-07	Needleman RB, et al.  (1984) MAL6 of Saccharomyces: a complex genetic locus containing three genes required for maltose fermentation. Proc Natl Acad Sci U S A 81(9):2811-5	SGD
trehalose transport	IDA: Inferred from Direct Assay Assigned on 2003-04-29	Stambuk BU and de Araujo PS  (2001) Kinetics of active alpha-glucoside transport in Saccharomyces cerevisiae. FEMS Yeast Res 1(1):73-8	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
plasma membrane	ISS: Inferred from Sequence or structural Similarity with SGD:HXT1, SGD:GAL2, SGD:HXT2, SGD:MAL61, SGD:SNF3, SGD:PHO84 Assigned on 2008-04-07	Han EK, et al.  (1995) Characterization of AGT1 encoding a general alpha-glucoside transporter from Saccharomyces. Mol Microbiol 17(6):1093-107	SGD
	ISS: Inferred from Sequence or structural Similarity with SGD:MAL61 Assigned on 2008-04-07	Gadura N and Michels CA  (2006) Sequences in the N-terminal cytoplasmic domain of Saccharomyces cerevisiae maltose permease are required for vacuolar degradation but not glucose-induced internalization. Curr Genet 50(2):101-14	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
substrate-specific transmembrane transporter activity	IEA: Inferred from Electronic Annotation with EBI:IPR003663 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
symporter activity	IEA: Inferred from Electronic Annotation with EBI:KW-0769 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transmembrane transporter activity	IEA: Inferred from Electronic Annotation with EBI:IPR005828 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
carbohydrate transport	IEA: Inferred from Electronic Annotation with EBI:KW-0762 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
maltose metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0462 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transmembrane transport	IEA: Inferred from Electronic Annotation with EBI:IPR005828, EBI:IPR003663 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:IPR005828 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:IPR003663 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0162 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).