This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-07-21    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IDA: Inferred from Direct Assay Assigned on 2009-08-31	Kassavetis GA, et al.  (1997) Domains of the Brf component of RNA polymerase III transcription factor IIIB (TFIIIB): functions in assembly of TFIIIB-DNA complexes and recruitment of RNA polymerase to the promoter. Mol Cell Biol 17(9):5299-306	SGD
RNA polymerase III core binding	IDA: Inferred from Direct Assay Assigned on 2011-07-21 IPI: Inferred from Physical Interaction Assigned on 2011-07-21	Kassavetis GA, et al.  (1998) A post-recruitment function for the RNA polymerase III transcription-initiation factor IIIB. Proc Natl Acad Sci U S A 95(16):9196-201	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-07-21 IPI: Inferred from Physical Interaction with SGD:RPC34 Assigned on 2011-07-21	Khoo B, et al.  (1994) Conserved functional domains of the RNA polymerase III general transcription factor BRF. Genes Dev 8(23):2879-90	SGD
TFIIIB-type transcription factor activity	IC: Inferred By Curator from DNA-dependent transcriptional open complex formation Assigned on 2011-07-21	Kassavetis GA, et al.  (2001) The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening. EMBO J 20(11):2823-34	SGD
	IC: Inferred By Curator from TFIIIC-class transcription factor binding Assigned on 2011-07-21	Liao Y, et al.  (2003) The Brf1 and Bdp1 subunits of transcription factor TFIIIB bind to overlapping sites in the tetratricopeptide repeats of Tfc4. J Biol Chem 278(45):44467-74	SGD
	IC: Inferred By Curator from transcription factor TFIIIB complex Assigned on 2011-07-21	Kassavetis GA, et al.  (1992) The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB. Cell 71(6):1055-64	SGD
	IC: Inferred By Curator from RNA polymerase III core binding Assigned on 2011-07-21	Khoo B, et al.  (1994) Conserved functional domains of the RNA polymerase III general transcription factor BRF. Genes Dev 8(23):2879-90	SGD
TFIIIC-class transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2011-07-21 IPI: Inferred from Physical Interaction with SGD:TFC4 Assigned on 2011-07-21	Liao Y, et al.  (2003) The Brf1 and Bdp1 subunits of transcription factor TFIIIB bind to overlapping sites in the tetratricopeptide repeats of Tfc4. J Biol Chem 278(45):44467-74	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-dependent transcriptional open complex formation	IMP: Inferred from Mutant Phenotype Assigned on 2011-07-21	Kassavetis GA, et al.  (2001) The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening. EMBO J 20(11):2823-34	SGD
negative regulation of protein homodimerization activity	IDA: Inferred from Direct Assay Assigned on 2009-09-14	Alexander DE, et al.  (2004) Inhibition of TATA binding protein dimerization by RNA polymerase III transcription initiation factor Brf1. J Biol Chem 279(31):32401-6	SGD
RNA polymerase III transcriptional preinitiation complex assembly	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-08 IDA: Inferred from Direct Assay Assigned on 2009-09-08	Kassavetis GA, et al.  (2001) The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening. EMBO J 20(11):2823-34	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-08	Kassavetis GA, et al.  (1998) Functional and structural organization of Brf, the TFIIB-related component of the RNA polymerase III transcription initiation complex. Mol Cell Biol 18(9):5587-99	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-08	Huet J, et al.  (1997) A cryptic DNA binding domain at the COOH terminus of TFIIIB70 affects formation, stability, and function of preinitiation complexes. J Biol Chem 272(29):18341-9	SGD
transposon integration	IDA: Inferred from Direct Assay Assigned on 2009-09-08	Yieh L, et al.  (2000) The Brf and TATA-binding protein subunits of the RNA polymerase III transcription factor IIIB mediate position-specific integration of the gypsy-like element, Ty3. J Biol Chem 275(38):29800-7	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
transcription factor TFIIIB complex	IDA: Inferred from Direct Assay Assigned on 2009-08-31	Buratowski S and Zhou H  (1992) A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB. Cell 71(2):221-30	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-08-31	Kassavetis GA, et al.  (1992) The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB. Cell 71(6):1055-64	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for BRF1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
TBP-class protein binding	IEA: Inferred from Electronic Annotation with EBI:IPR013150 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
zinc ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR000812, EBI:IPR013137, EBI:IPR011665 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-dependent transcription, initiation	IEA: Inferred from Electronic Annotation with EBI:IPR000812 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
positive regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:IPR011665 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:IPR000812, EBI:IPR013137 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR011665 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).