This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-04-11    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
disulfide oxidoreductase activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-01-11	Gan ZR  (1991) Yeast thioredoxin genes. J Biol Chem 266(3):1692-6	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell redox homeostasis	TAS: Traceable Author Statement Assigned on 2002-04-11	Grant CM  (2001) Role of the glutathione/glutaredoxin and thioredoxin systems in yeast growth and response to stress conditions. Mol Microbiol 39(3):533-41	SGD
cellular response to oxidative stress	ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18	Gan ZR  (1991) Yeast thioredoxin genes. J Biol Chem 266(3):1692-6	SGD
ER to Golgi vesicle-mediated transport	IDA: Inferred from Direct Assay Assigned on 2005-06-21	Spang A and Schekman R  (1998) Reconstitution of retrograde transport from the Golgi to the ER in vitro. J Cell Biol 143(3):589-99	SGD
protein deglutathionylation	IGI: Inferred from Genetic Interaction with SGD:TRX1 Assigned on 2010-02-03 IDA: Inferred from Direct Assay Assigned on 2010-02-03	Greetham D, et al.  (2010) Thioredoxins function as deglutathionylase enzymes in the yeast Saccharomyces cerevisiae. BMC Biochem 11():3	SGD
retrograde vesicle-mediated transport, Golgi to ER	IDA: Inferred from Direct Assay Assigned on 2005-06-21	Spang A and Schekman R  (1998) Reconstitution of retrograde transport from the Golgi to the ER in vitro. J Cell Biol 143(3):589-99	SGD
sulfate assimilation	IGI: Inferred from Genetic Interaction with SGD:TRX1 Assigned on 2005-06-17	Muller EG  (1991) Thioredoxin deficiency in yeast prolongs S phase and shortens the G1 interval of the cell cycle. J Biol Chem 266(14):9194-202	SGD
vacuole fusion, non-autophagic	IDA: Inferred from Direct Assay Assigned on 2002-04-11	Xu Z, et al.  (1998) LMA1 binds to vacuoles at Sec18p (NSF), transfers upon ATP hydrolysis to a t-SNARE (Vam3p) complex, and is released during fusion. Cell 93(7):1125-34	SGD
vacuole inheritance	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Xu Z and Wickner W  (1996) Thioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae. J Cell Biol 132(5):787-94	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Xu Z, et al.  (1997) A heterodimer of thioredoxin and I(B)2 cooperates with Sec18p (NSF) to promote yeast vacuole inheritance. J Cell Biol 136(2):299-306	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Xu Z and Wickner W  (1996) Thioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae. J Cell Biol 132(5):787-94	SGD
fungal-type vacuole	IPI: Inferred from Physical Interaction Assigned on 2002-04-11	Xu Z, et al.  (1998) LMA1 binds to vacuoles at Sec18p (NSF), transfers upon ATP hydrolysis to a t-SNARE (Vam3p) complex, and is released during fusion. Cell 93(7):1125-34	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for TRX2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
electron carrier activity	IEA: Inferred from Electronic Annotation with EBI:IPR005746 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein disulfide oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:IPR005746 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell redox homeostasis	IEA: Inferred from Electronic Annotation with EBI:IPR005746, EBI:IPR013766, EBI:IPR017937 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
deoxyribonucleotide biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:KW-0215 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
electron transport chain	IEA: Inferred from Electronic Annotation with EBI:KW-0249 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
glycerol ether metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR005746 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
Golgi apparatus	IEA: Inferred from Electronic Annotation with EBI:KW-0333 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
Golgi membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0134 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).