This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-12-18    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
formate-tetrahydrofolate ligase activity	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-18	Song JM and Rabinowitz JC  (1993) Function of yeast cytoplasmic C1-tetrahydrofolate synthase. Proc Natl Acad Sci U S A 90(7):2636-40	SGD
methenyltetrahydrofolate cyclohydrolase activity	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-18	Song JM and Rabinowitz JC  (1993) Function of yeast cytoplasmic C1-tetrahydrofolate synthase. Proc Natl Acad Sci U S A 90(7):2636-40	SGD
methylenetetrahydrofolate dehydrogenase (NADP+) activity	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-18	Song JM and Rabinowitz JC  (1993) Function of yeast cytoplasmic C1-tetrahydrofolate synthase. Proc Natl Acad Sci U S A 90(7):2636-40	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
folic acid-containing compound metabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-19	Lomax CA, et al.  (1971) New mutant types at the ade3 locus of Saccharomyces cerevisiae. J Bacteriol 107(1):1-7	SGD
purine nucleobase biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-27	Song JM and Rabinowitz JC  (1993) Function of yeast cytoplasmic C1-tetrahydrofolate synthase. Proc Natl Acad Sci U S A 90(7):2636-40	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR020628, EBI:IPR000559 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR020631, EBI:IPR000672, EBI:IPR020867, EBI:IPR020630 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0511 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
formate-tetrahydrofolate ligase activity	IEA: Inferred from Electronic Annotation with EBI:IPR020628, EBI:IPR000559 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:6.3.4.3 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ligase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0436 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
methenyltetrahydrofolate cyclohydrolase activity	IEA: Inferred from Electronic Annotation with IUBMB:3.5.4.9 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
methylenetetrahydrofolate dehydrogenase (NADP+) activity	IEA: Inferred from Electronic Annotation with EBI:IPR020631, EBI:IPR000672, EBI:IPR020630 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:1.5.1.5 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR016040 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular amino acid biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:KW-0028 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
folic acid-containing compound biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:IPR020631, EBI:IPR020628, EBI:IPR000672, EBI:IPR020867, EBI:IPR020630, EBI:IPR000559 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
histidine biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:KW-0368 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0511 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
methionine biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:KW-0486 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
one-carbon metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0554 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidation-reduction process	IEA: Inferred from Electronic Annotation with EBI:IPR020631, EBI:IPR000672, EBI:IPR020867, EBI:IPR020630 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
purine nucleotide biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:KW-0658 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
tetrahydrofolate interconversion	IEA: Inferred from Electronic Annotation with UniPathway:UPA00193 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).