This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-12-02    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
core RNA polymerase II binding transcription factor activity	IDA: Inferred from Direct Assay Assigned on 2011-11-15	Rani PG, et al.  (2004) RNA polymerase II (Pol II)-TFIIF and Pol II-mediator complexes: the major stable Pol II complexes and their activity in transcription initiation and reinitiation. Mol Cell Biol 24(4):1709-20	SGD
contributes_to core RNA polymerase II recruiting transcription factor activity	IC: Inferred By Curator from transcription factor TFIIF complex Assigned on 2011-10-05	Henry NL, et al.  (1994) TFIIF-TAF-RNA polymerase II connection. Genes Dev 8(23):2868-78	SGD
protein phosphatase activator activity	IDA: Inferred from Direct Assay Assigned on 2011-08-26	Kops O, et al.  (2002) Pin1 modulates the dephosphorylation of the RNA polymerase II C-terminal domain by yeast Fcp1. FEBS Lett 513(2-3):305-11	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
dephosphorylation of RNA polymerase II C-terminal domain	IGI: Inferred from Genetic Interaction with SGD:FCP1 Assigned on 2011-08-26	Kobor MS, et al.  (2000) A motif shared by TFIIF and TFIIB mediates their interaction with the RNA polymerase II carboxy-terminal domain phosphatase Fcp1p in Saccharomyces cerevisiae. Mol Cell Biol 20(20):7438-49	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-08-26	Kops O, et al.  (2002) Pin1 modulates the dephosphorylation of the RNA polymerase II C-terminal domain by yeast Fcp1. FEBS Lett 513(2-3):305-11	SGD
promoter clearance from RNA polymerase II promoter	IC: Inferred By Curator from transcription factor TFIIF complex Assigned on 2011-12-02	Henry NL, et al.  (1994) TFIIF-TAF-RNA polymerase II connection. Genes Dev 8(23):2868-78	SGD
regulation of protein serine/threonine phosphatase activity	IDA: Inferred from Direct Assay Assigned on 2011-08-26	Kops O, et al.  (2002) Pin1 modulates the dephosphorylation of the RNA polymerase II C-terminal domain by yeast Fcp1. FEBS Lett 513(2-3):305-11	SGD
RNA polymerase II transcriptional preinitiation complex assembly	IC: Inferred By Curator from transcription factor TFIIF complex Assigned on 2011-11-22	Henry NL, et al.  (1994) TFIIF-TAF-RNA polymerase II connection. Genes Dev 8(23):2868-78	SGD
transcription elongation from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2011-11-15 IDA: Inferred from Direct Assay Assigned on 2011-11-15	Khaperskyy DA, et al.  (2008) Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization. Mol Cell Biol 28(11):3757-66	SGD
transcription initiation from RNA polymerase II promoter	IDA: Inferred from Direct Assay Assigned on 2011-11-18	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-11-18 IDA: Inferred from Direct Assay Assigned on 2011-11-18	Khaperskyy DA, et al.  (2008) Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization. Mol Cell Biol 28(11):3757-66	SGD
transcriptional start site selection at RNA polymerase II promoter	IGI: Inferred from Genetic Interaction with SGD:SUA7, SGD:RPO21 Assigned on 2011-12-02	Freire-Picos MA, et al.  (2005) Evidence that the Tfg1/Tfg2 dimer interface of TFIIF lies near the active center of the RNA polymerase II initiation complex. Nucleic Acids Res 33(16):5045-52	SGD
	IGI: Inferred from Genetic Interaction with SGD:RPO21 Assigned on 2011-12-02 IMP: Inferred from Mutant Phenotype Assigned on 2011-12-02	Majovski RC, et al.  (2005) A functional role for the switch 2 region of yeast RNA polymerase II in transcription start site utilization and abortive initiation. J Biol Chem 280(41):34917-23	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-02	Khaperskyy DA, et al.  (2008) Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization. Mol Cell Biol 28(11):3757-66	SGD
	IGI: Inferred from Genetic Interaction with SGD:SUA7, SGD:RPB9 Assigned on 2011-12-02 IMP: Inferred from Mutant Phenotype Assigned on 2011-12-02	Ghazy MA, et al.  (2004) Amino acid substitutions in yeast TFIIF confer upstream shifts in transcription initiation and altered interaction with RNA polymerase II. Mol Cell Biol 24(24):10975-85	SGD
	IGI: Inferred from Genetic Interaction with SGD:SUA7 Assigned on 2011-12-02	Sun ZW and Hampsey M  (1995) Identification of the gene (SSU71/TFG1) encoding the largest subunit of transcription factor TFIIF as a suppressor of a TFIIB mutation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 92(8):3127-31	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
transcription factor TFIIF complex	IDA: Inferred from Direct Assay Assigned on 2011-08-26	Krogan NJ, et al.  (2002) RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Mol Cell Biol 22(20):6979-92	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-08-26	Henry NL, et al.  (1994) TFIIF-TAF-RNA polymerase II connection. Genes Dev 8(23):2868-78	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for TFG1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR011039 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR008851 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
positive regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:IPR008851 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription initiation from RNA polymerase II promoter	IEA: Inferred from Electronic Annotation with EBI:IPR011039 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR008851 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).