This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2009-11-11    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ubiquitin-protein ligase activity	IPI: Inferred from Physical Interaction with SGD:CUP9 Assigned on 2009-11-11	Xia Z, et al.  (2008) Substrate-binding Sites of UBR1, the Ubiquitin Ligase of the N-end Rule Pathway. J Biol Chem 283(35):24011-28	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-01-05 IPI: Inferred from Physical Interaction with SGD:RAD6 Assigned on 2009-11-11	Xie Y and Varshavsky A  (1999) The E2-E3 interaction in the N-end rule pathway: the RING-H2 finger of E3 is required for the synthesis of multiubiquitin chain. EMBO J 18(23):6832-44	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-26	Heck JW, et al.  (2010) Cytoplasmic protein quality control degradation mediated by parallel actions of the E3 ubiquitin ligases Ubr1 and San1. Proc Natl Acad Sci U S A 107(3):1106-11	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2013-02-25	Khosrow-Khavar F, et al.  (2012) The yeast ubr1 ubiquitin ligase participates in a prominent pathway that targets cytosolic thermosensitive mutants for degradation. G3 (Bethesda) 2(5):619-28	SGD
protein polyubiquitination	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-11	Xie Y and Varshavsky A  (1999) The E2-E3 interaction in the N-end rule pathway: the RING-H2 finger of E3 is required for the synthesis of multiubiquitin chain. EMBO J 18(23):6832-44	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-11	Bartel B, et al.  (1990) The recognition component of the N-end rule pathway. EMBO J 9(10):3179-89	SGD
regulation of dipeptide transport	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-11	Byrd C, et al.  (1998) The N-end rule pathway controls the import of peptides through degradation of a transcriptional repressor. EMBO J 17(1):269-77	SGD
ubiquitin-dependent protein catabolic process via the N-end rule pathway	IMP: Inferred from Mutant Phenotype Assigned on 2010-02-05	Bartel B, et al.  (1990) The recognition component of the N-end rule pathway. EMBO J 9(10):3179-89	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IGI: Inferred from Genetic Interaction with SGD:SAN1 Assigned on 2010-02-05 IMP: Inferred from Mutant Phenotype Assigned on 2010-02-05	Heck JW, et al.  (2010) Cytoplasmic protein quality control degradation mediated by parallel actions of the E3 ubiquitin ligases Ubr1 and San1. Proc Natl Acad Sci U S A 107(3):1106-11	SGD
colocalizes_with proteasome regulatory particle, base subcomplex	IPI: Inferred from Physical Interaction with SGD:RPN2, SGD:RPT6, SGD:RPT1 Assigned on 2009-11-11	Xie Y and Varshavsky A  (2000) Physical association of ubiquitin ligases and the 26S proteasome. Proc Natl Acad Sci U S A 97(6):2497-502	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for UBR1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ligase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0436 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ubiquitin-protein ligase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003126 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
zinc ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR001841, EBI:IPR003126 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR003769 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein ubiquitination	IEA: Inferred from Electronic Annotation with UniPathway:UPA00143 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).