This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-03-24    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
chaperone binding	IPI: Inferred from Physical Interaction with SGD:SIS1 Assigned on 2012-12-03	Malinovska L, et al.  (2012) Molecular chaperones and stress-inducible protein-sorting factors coordinate the spatiotemporal distribution of protein aggregates. Mol Biol Cell 23(16):3041-56	SGD
SNARE binding	IDA: Inferred from Direct Assay Assigned on 2011-01-31 IPI: Inferred from Physical Interaction with SGD:SNC1 Assigned on 2011-01-31	Kama R, et al.  (2007) Btn2, a hook1 ortholog and potential batten disease-related protein, mediates late endosome-Golgi protein sorting in yeast. Mol Cell Biol 27(2):605-21	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
amino acid transport	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	Kim Y, et al.  (2005) Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae. Eukaryot Cell 4(2):281-8	SGD
intracellular protein transport	IMP: Inferred from Mutant Phenotype Assigned on 2012-12-03 IPI: Inferred from Physical Interaction with SGD:SIS1 Assigned on 2012-12-03	Malinovska L, et al.  (2012) Molecular chaperones and stress-inducible protein-sorting factors coordinate the spatiotemporal distribution of protein aggregates. Mol Biol Cell 23(16):3041-56	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-03-24 IPI: Inferred from Physical Interaction Assigned on 2003-03-24	Chattopadhyay S, et al.  (2003) The yeast model for Batten disease: a role for Btn2p in the trafficking of the Golgi-associated vesicular targeting protein, Yif1p. Biochem Biophys Res Commun 302(3):534-8	SGD
protein folding	IGI: Inferred from Genetic Interaction with SGD:SIS1 Assigned on 2012-12-03 IMP: Inferred from Mutant Phenotype Assigned on 2012-12-03 IPI: Inferred from Physical Interaction with SGD:SIS1 Assigned on 2012-12-03	Malinovska L, et al.  (2012) Molecular chaperones and stress-inducible protein-sorting factors coordinate the spatiotemporal distribution of protein aggregates. Mol Biol Cell 23(16):3041-56	SGD
protein localization to nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2012-12-03 IPI: Inferred from Physical Interaction with SGD:SRP1 Assigned on 2012-12-03	Malinovska L, et al.  (2012) Molecular chaperones and stress-inducible protein-sorting factors coordinate the spatiotemporal distribution of protein aggregates. Mol Biol Cell 23(16):3041-56	SGD
regulation of pH	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	Kim Y, et al.  (2005) Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae. Eukaryot Cell 4(2):281-8	SGD
	IGI: Inferred from Genetic Interaction Assigned on 2001-02-06 IMP: Inferred from Mutant Phenotype Assigned on 2001-02-06	Chattopadhyay S, et al.  (2000) The yeast model for batten disease: mutations in BTN1, BTN2, and HSP30 alter pH homeostasis. J Bacteriol 182(22):6418-23	SGD
retrograde transport, endosome to Golgi	IMP: Inferred from Mutant Phenotype Assigned on 2006-11-29	Kama R, et al.  (2007) Btn2, a hook1 ortholog and potential batten disease-related protein, mediates late endosome-Golgi protein sorting in yeast. Mol Cell Biol 27(2):605-21	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2012-12-03	Malinovska L, et al.  (2012) Molecular chaperones and stress-inducible protein-sorting factors coordinate the spatiotemporal distribution of protein aggregates. Mol Biol Cell 23(16):3041-56	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-02-06	Chattopadhyay S, et al.  (2000) The yeast model for batten disease: mutations in BTN1, BTN2, and HSP30 alter pH homeostasis. J Bacteriol 182(22):6418-23	SGD
late endosome	IDA: Inferred from Direct Assay Assigned on 2006-11-29	Kama R, et al.  (2007) Btn2, a hook1 ortholog and potential batten disease-related protein, mediates late endosome-Golgi protein sorting in yeast. Mol Cell Biol 27(2):605-21	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2013-01-20	Malinovska L, et al.  (2012) Molecular chaperones and stress-inducible protein-sorting factors coordinate the spatiotemporal distribution of protein aggregates. Mol Biol Cell 23(16):3041-56	SGD
perinuclear region of cytoplasm	IDA: Inferred from Direct Assay Assigned on 2008-10-14	Kryndushkin DS, et al.  (2008) Curing of the [URE3] prion by Btn2p, a Batten disease-related protein. EMBO J 27(20):2725-35	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for BTN2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
endosome	IEA: Inferred from Electronic Annotation with EBI:KW-0967 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
late endosome	IEA: Inferred from Electronic Annotation with EBI:SL-0152 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).