This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-01-22    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to microtubule binding	IDA: Inferred from Direct Assay Assigned on 2011-01-18	Asbury CL, et al.  (2006) The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement. Proc Natl Acad Sci U S A 103(26):9873-8	SGD
structural constituent of cytoskeleton	IPI: Inferred from Physical Interaction Assigned on 2002-10-08	Cheeseman IM, et al.  (2001) Implication of a novel multiprotein Dam1p complex in outer kinetochore function. J Cell Biol 155(7):1137-45	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mitotic spindle organization in nucleus	IGI: Inferred from Genetic Interaction Assigned on 2002-10-08	Cheeseman IM, et al.  (2001) Implication of a novel multiprotein Dam1p complex in outer kinetochore function. J Cell Biol 155(7):1137-45	SGD
regulation of microtubule polymerization or depolymerization	IDA: Inferred from Direct Assay Assigned on 2005-03-28 IPI: Inferred from Physical Interaction Assigned on 2005-03-28	Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
condensed nuclear chromosome kinetochore	IPI: Inferred from Physical Interaction Assigned on 2002-10-08	Cheeseman IM, et al.  (2001) Implication of a novel multiprotein Dam1p complex in outer kinetochore function. J Cell Biol 155(7):1137-45	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-03-07	He X, et al.  (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206	SGD
DASH complex	IDA: Inferred from Direct Assay Assigned on 2005-03-28 IPI: Inferred from Physical Interaction Assigned on 2005-03-28	Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-01-22 IPI: Inferred from Physical Interaction Assigned on 2004-01-22	Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-03-28 IPI: Inferred from Physical Interaction Assigned on 2005-03-28	Miranda JJ, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43	SGD
kinetochore microtubule	IDA: Inferred from Direct Assay Assigned on 2002-06-02	Kang Js JS, et al.  (2001) Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation. J Cell Biol 155(5):763-74	SGD
spindle	IPI: Inferred from Physical Interaction Assigned on 2002-10-08	Cheeseman IM, et al.  (2001) Implication of a novel multiprotein Dam1p complex in outer kinetochore function. J Cell Biol 155(7):1137-45	SGD
spindle midzone	IDA: Inferred from Direct Assay Assigned on 2005-04-07	Buvelot S, et al.  (2003) The budding yeast Ipl1/Aurora protein kinase regulates mitotic spindle disassembly. J Cell Biol 160(3):329-39	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for DAM1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromosome segregation	IEA: Inferred from Electronic Annotation with EBI:KW-0159 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitosis	IEA: Inferred from Electronic Annotation with EBI:KW-0498 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitotic spindle organization in nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR013962 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome	IEA: Inferred from Electronic Annotation with EBI:KW-0158 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromosome, centromeric region	IEA: Inferred from Electronic Annotation with EBI:KW-0137 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
condensed chromosome kinetochore	IEA: Inferred from Electronic Annotation with EBI:SL-0149 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoskeleton	IEA: Inferred from Electronic Annotation with EBI:KW-0206 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DASH complex	IEA: Inferred from Electronic Annotation with EBI:IPR013962 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
kinetochore	IEA: Inferred from Electronic Annotation with EBI:KW-0995 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
microtubule	IEA: Inferred from Electronic Annotation with EBI:KW-0493 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitotic spindle	IEA: Inferred from Electronic Annotation with EBI:IPR013962 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
spindle	IEA: Inferred from Electronic Annotation with EBI:SL-0251 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).