This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-28    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ubiquitin-protein ligase activity	IDA: Inferred from Direct Assay Assigned on 2003-07-08	Michel JJ, et al.  (2003) A role for Saccharomyces cerevisiae Cul8 ubiquitin ligase in proper anaphase progression. J Biol Chem 278(25):22828-37	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein ubiquitination involved in ubiquitin-dependent protein catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-21	Ribar B, et al.  (2007) ELA1 and CUL3 are required along with ELC1 for RNA polymerase II polyubiquitylation and degradation in DNA-damaged yeast cells. Mol Cell Biol 27(8):3211-6	SGD
ubiquitin-dependent protein catabolic process	IC: Inferred By Curator from ubiquitin-protein ligase activity Assigned on 2003-10-06	Michel JJ, et al.  (2003) A role for Saccharomyces cerevisiae Cul8 ubiquitin ligase in proper anaphase progression. J Biol Chem 278(25):22828-37	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-10-06	Lammer D, et al.  (1998) Modification of yeast Cdc53p by the ubiquitin-related protein rub1p affects function of the SCFCdc4 complex. Genes Dev 12(7):914-26	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
Cul3-RING ubiquitin ligase complex	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-21	Ribar B, et al.  (2007) ELA1 and CUL3 are required along with ELC1 for RNA polymerase II polyubiquitylation and degradation in DNA-damaged yeast cells. Mol Cell Biol 27(8):3211-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-05-21	Gillette TG, et al.  (2006) Distinct functions of the ubiquitin-proteasome pathway influence nucleotide excision repair. EMBO J 25(11):2529-38	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ubiquitin protein ligase binding	IEA: Inferred from Electronic Annotation with EBI:IPR019559, EBI:IPR016158, EBI:IPR001373, EBI:IPR016157 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein ubiquitination	IEA: Inferred from Electronic Annotation with UniPathway:UPA00143 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
ubiquitin-dependent protein catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR019559, EBI:IPR016158, EBI:IPR016157, EBI:IPR001373 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cullin-RING ubiquitin ligase complex	IEA: Inferred from Electronic Annotation with EBI:IPR019559, EBI:IPR016158, EBI:IPR016157, EBI:IPR001373 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).