This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-01-28    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
plus-end-directed microtubule motor activity	IDA: Inferred from Direct Assay Assigned on 2009-02-27	Varga V, et al.  (2006) Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol 8(9):957-62	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-02-27	Gupta ML Jr, et al.  (2006) Plus end-specific depolymerase activity of Kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle. Nat Cell Biol 8(9):913-23	SGD
tubulin-dependent ATPase activity	IDA: Inferred from Direct Assay Assigned on 2009-03-04	Gupta ML Jr, et al.  (2006) Plus end-specific depolymerase activity of Kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle. Nat Cell Biol 8(9):913-23	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
establishment of mitotic spindle orientation	IGI: Inferred from Genetic Interaction Assigned on 2004-01-28 IMP: Inferred from Mutant Phenotype Assigned on 2004-01-28	Cottingham FR and Hoyt MA  (1997) Mitotic spindle positioning in Saccharomyces cerevisiae is accomplished by antagonistically acting microtubule motor proteins. J Cell Biol 138(5):1041-53	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-02-27	Gupta ML Jr, et al.  (2006) Plus end-specific depolymerase activity of Kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle. Nat Cell Biol 8(9):913-23	SGD
mitotic spindle disassembly	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-07	Straight AF, et al.  (1998) Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast. J Cell Biol 143(3):687-94	SGD
mitotic spindle organization in nucleus	IGI: Inferred from Genetic Interaction Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Cottingham FR, et al.  (1999) Novel roles for saccharomyces cerevisiae mitotic spindle motors. J Cell Biol 147(2):335-50	SGD
nuclear migration along microtubule	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Miller RK, et al.  (1998) The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast. Mol Biol Cell 9(8):2051-68	SGD
plus-end specific microtubule depolymerization	IDA: Inferred from Direct Assay Assigned on 2009-03-04	Varga V, et al.  (2006) Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol 8(9):957-62	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-03-04	Gupta ML Jr, et al.  (2006) Plus end-specific depolymerase activity of Kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle. Nat Cell Biol 8(9):913-23	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasmic microtubule	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Miller RK, et al.  (1998) The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast. Mol Biol Cell 9(8):2051-68	SGD
kinesin complex	TAS: Traceable Author Statement Assigned on 2001-01-18	Winsor B and Schiebel E  (1997) Review: an overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton. Yeast 13(5):399-434	SGD
nuclear microtubule	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Miller RK, et al.  (1998) The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast. Mol Biol Cell 9(8):2051-68	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for KIP3

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR019821, EBI:IPR001752 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
microtubule motor activity	IEA: Inferred from Electronic Annotation with EBI:IPR019821, EBI:IPR001752 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
microtubule-based movement	IEA: Inferred from Electronic Annotation with EBI:IPR019821, EBI:IPR001752 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoskeleton	IEA: Inferred from Electronic Annotation with EBI:SL-0090 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0206 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
microtubule	IEA: Inferred from Electronic Annotation with EBI:KW-0493 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
microtubule associated complex	IEA: Inferred from Electronic Annotation with EBI:IPR001752 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).