This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-08-14    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to AMP-activated protein kinase activity	IGI: Inferred from Genetic Interaction with SGD:SIP1, SGD:GAL83 Assigned on 2011-04-07	Schmidt MC and McCartney RR  (2000) beta-subunits of Snf1 kinase are required for kinase function and substrate definition. EMBO J 19(18):4936-43	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular response to glucose starvation	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-16	Ashrafi K, et al.  (2000) Sip2p and its partner snf1p kinase affect aging in S. cerevisiae. Genes Dev 14(15):1872-85	SGD
invasive growth in response to glucose limitation	IGI: Inferred from Genetic Interaction with SGD:GAL83 Assigned on 2006-06-28	Vyas VK, et al.  (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8	SGD
protein phosphorylation	IGI: Inferred from Genetic Interaction with SGD:SIP1, SGD:GAL83 Assigned on 2007-03-08	Schmidt MC and McCartney RR  (2000) beta-subunits of Snf1 kinase are required for kinase function and substrate definition. EMBO J 19(18):4936-43	SGD
regulation of protein complex assembly	IGI: Inferred from Genetic Interaction with SGD:SIP1, SGD:GAL83 Assigned on 2007-03-09	Jiang R and Carlson M  (1997) The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex. Mol Cell Biol 17(4):2099-106	SGD
replicative cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-23	Ashrafi K, et al.  (2000) Sip2p and its partner snf1p kinase affect aging in S. cerevisiae. Genes Dev 14(15):1872-85	SGD
	IGI: Inferred from Genetic Interaction with SGD:FOB1 Assigned on 2007-03-09 IMP: Inferred from Mutant Phenotype Assigned on 2007-03-09	Lin SS, et al.  (2003) Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing. J Biol Chem 278(15):13390-7	SGD
signal transduction	IGI: Inferred from Genetic Interaction with SGD:SIP1, SGD:GAL83 Assigned on 2007-03-08	Schmidt MC and McCartney RR  (2000) beta-subunits of Snf1 kinase are required for kinase function and substrate definition. EMBO J 19(18):4936-43	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
AMP-activated protein kinase complex	IPI: Inferred from Physical Interaction with SGD:SNF1 Assigned on 2007-03-09	Yang X, et al.  (1994) A family of proteins containing a conserved domain that mediates interaction with the yeast SNF1 protein kinase complex. EMBO J 13(24):5878-86	SGD
	IPI: Inferred from Physical Interaction with SGD:SNF4, SGD:SNF1 Assigned on 2007-03-09	Jiang R and Carlson M  (1997) The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex. Mol Cell Biol 17(4):2099-106	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-03-12	Nath N, et al.  (2002) Purification and characterization of Snf1 kinase complexes containing a defined Beta subunit composition. J Biol Chem 277(52):50403-8	SGD
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-10-09	Vincent O, et al.  (2001) Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism. Genes Dev 15(9):1104-14	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2003-04-25	Lin SS, et al.  (2003) Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing. J Biol Chem 278(15):13390-7	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SIP2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
plasma membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0039 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-1003 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).