This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-06-21    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ARF GTPase activator activity	ISS: Inferred from Sequence or structural Similarity with SGD:GCS1 Assigned on 2010-01-08	Yaguchi S, et al.  (2007) Localization of Gts1p in cortical actin patches of yeast and its possible role in endocytosis. Eur J Cell Biol 86(5):275-85	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-12	Smaczynska-de Rooij II, et al.  (2008) Yeast Arf3p Modulates Plasma Membrane PtdIns(4,5)P2 Levels to Facilitate Endocytosis. Traffic 9(4):559-73	SGD
repressing transcription factor binding	IPI: Inferred from Physical Interaction with SGD:SFL1 Assigned on 2011-06-21	Shen H, et al.  (2006) The mechanism by which overexpression of Gts1p induces flocculation in a FLO8-inactive strain of the yeast Saccharomyces cerevisiae. FEMS Yeast Res 6(6):914-23	SGD
transcription factor binding transcription factor activity	IMP: Inferred from Mutant Phenotype Assigned on 2011-06-21 IPI: Inferred from Physical Interaction with SGD:SFL1 Assigned on 2011-06-21	Shen H, et al.  (2006) The mechanism by which overexpression of Gts1p induces flocculation in a FLO8-inactive strain of the yeast Saccharomyces cerevisiae. FEMS Yeast Res 6(6):914-23	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-13	Yaguchi S, et al.  (2007) Localization of Gts1p in cortical actin patches of yeast and its possible role in endocytosis. Eur J Cell Biol 86(5):275-85	SGD
negative regulation of phosphatidylinositol biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-12	Smaczynska-de Rooij II, et al.  (2008) Yeast Arf3p Modulates Plasma Membrane PtdIns(4,5)P2 Levels to Facilitate Endocytosis. Traffic 9(4):559-73	SGD
positive regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2011-06-21	Yaguchi S and Tsurugi K  (2003) Gts1p activates SNF1-dependent derepression of HSP104 and TPS1 in the stationary phase of yeast growth. J Biol Chem 278(32):29760-8	SGD
regulation of transcription from RNA polymerase II promoter	IPI: Inferred from Physical Interaction with SGD:SRB5 Assigned on 2011-06-21	Shen H, et al.  (2006) The mechanism by which overexpression of Gts1p induces flocculation in a FLO8-inactive strain of the yeast Saccharomyces cerevisiae. FEMS Yeast Res 6(6):914-23	SGD
ultradian rhythm	IMP: Inferred from Mutant Phenotype Assigned on 2002-12-12	Saito T, et al.  (2002) Regulation of the Gts1p level by the ubiquitination system to maintain metabolic oscillations in the continuous culture of yeast. J Biol Chem 277(37):33624-31	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2004-07-13	Wang J, et al.  (2001) Evidence for the involvement of the GTS1 gene product in the regulation of biological rhythms in the continuous culture of the yeast Saccharomyces cerevisiae. FEBS Lett 489(1):81-6	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2004-07-13	Wang J, et al.  (1998) The GTS1 gene product influences the ultradian oscillation of glycolysis in cell suspension of the yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun 244(1):239-42	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
actin cortical patch	IDA: Inferred from Direct Assay Assigned on 2007-09-13	Yaguchi S, et al.  (2007) Localization of Gts1p in cortical actin patches of yeast and its possible role in endocytosis. Eur J Cell Biol 86(5):275-85	SGD
cytoplasm	IPI: Inferred from Physical Interaction Assigned on 2001-01-18	Kawabata K, et al.  (1999) Protein interactions of Gts1p of Saccharomyces cerevisiae throughout a region similar to a cytoplasmic portion of some ATP-binding cassette transporters. Eur J Biochem 259(1-2):112-9	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2008-04-09	Yaguchi S, et al.  (2007) Localization of Gts1p in cortical actin patches of yeast and its possible role in endocytosis. Eur J Cell Biol 86(5):275-85	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-10-30	Iha H, et al.  (2001) Functional correlation between the nuclear localization of Fht1p and its flocculation and heat tolerance activities in budding yeast Saccharomyces cerevisiae. J Biomed Sci 8(5):416-20	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for GTS1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ARF GTPase activator activity	IEA: Inferred from Electronic Annotation with EBI:IPR001164 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
zinc ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR001164 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of ARF GTPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR001164 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).