This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-11-14    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
anaphase-promoting complex binding	IDA: Inferred from Direct Assay Assigned on 2011-11-14	Kraft C, et al.  (2005) The WD40 propeller domain of Cdh1 functions as a destruction box receptor for APC/C substrates. Mol Cell 18(5):543-53	SGD
ubiquitin-protein ligase activator activity	IDA: Inferred from Direct Assay Assigned on 2011-11-14	Matyskiela ME and Morgan DO  (2009) Analysis of activator-binding sites on the APC/C supports a cooperative substrate-binding mechanism. Mol Cell 34(1):68-80	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
activation of anaphase-promoting complex activity involved in meiotic cell cycle	IMP: Inferred from Mutant Phenotype Assigned on 2011-11-14	Mallory MJ, et al.  (2007) Meiosis-specific destruction of the Ume6p repressor by the Cdc20-directed APC/C. Mol Cell 27(6):951-61	SGD
activation of mitotic anaphase-promoting complex activity	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-04	Visintin R, et al.  (1997) CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis. Science 278(5337):460-3	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-04	Schott EJ and Hoyt MA  (1998) Dominant alleles of Saccharomyces cerevisiae CDC20 reveal its role in promoting anaphase. Genetics 148(2):599-610	SGD
cyclin catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2011-11-14	Wasch R and Cross FR  (2002) APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit. Nature 418(6897):556-62	SGD
meiotic anaphase I	IMP: Inferred from Mutant Phenotype Assigned on 2011-11-14	Salah SM and Nasmyth K  (2000) Destruction of the securin Pds1p occurs at the onset of anaphase during both meiotic divisions in yeast. Chromosoma 109(1-2):27-34	SGD
positive regulation of mitotic metaphase/anaphase transition	IMP: Inferred from Mutant Phenotype Assigned on 2011-11-14	Shirayama M, et al.  (1999) APC(Cdc20) promotes exit from mitosis by destroying the anaphase inhibitor Pds1 and cyclin Clb5. Nature 402(6758):203-7	SGD
positive regulation of protein catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2011-11-14	Visintin R, et al.  (1997) CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis. Science 278(5337):460-3	SGD
spindle checkpoint	IPI: Inferred from Physical Interaction with SGD:MAD2 Assigned on 2011-11-14	Hwang LH, et al.  (1998) Budding yeast Cdc20: a target of the spindle checkpoint. Science 279(5353):1041-4	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
anaphase-promoting complex	IPI: Inferred from Physical Interaction with SGD:CDC23 Assigned on 2007-07-11	Lim HH, et al.  (1998) Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and Clb2 during M phase in budding yeast. Curr Biol 8(4):231-4	SGD
mitotic checkpoint complex	IDA: Inferred from Direct Assay Assigned on 2007-10-04 IPI: Inferred from Physical Interaction with SGD:BUB3, SGD:MAD3, SGD:MAD2 Assigned on 2007-10-01	Fraschini R, et al.  (2001) Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. EMBO J 20(23):6648-59	SGD
	IPI: Inferred from Physical Interaction with SGD:MAD3, SGD:MAD2 Assigned on 2007-10-01	Hardwick KG, et al.  (2000) MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p. J Cell Biol 148(5):871-82	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitosis	IEA: Inferred from Electronic Annotation with EBI:KW-0498 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).