This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-03-12    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to AMP-activated protein kinase activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-09	McCartney RR and Schmidt MC  (2001) Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit. J Biol Chem 276(39):36460-6	SGD
	IGI: Inferred from Genetic Interaction with SGD:SNF1 Assigned on 2007-03-09 IMP: Inferred from Mutant Phenotype Assigned on 2007-03-09	Celenza JL and Carlson M  (1989) Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein. Mol Cell Biol 9(11):5034-44	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-09	Woods A, et al.  (1994) Yeast SNF1 is functionally related to mammalian AMP-activated protein kinase and regulates acetyl-CoA carboxylase in vivo. J Biol Chem 269(30):19509-15	SGD
protein serine/threonine kinase activator activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-09	McCartney RR and Schmidt MC  (2001) Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit. J Biol Chem 276(39):36460-6	SGD
	IGI: Inferred from Genetic Interaction with SGD:SNF1 Assigned on 2007-03-08 IMP: Inferred from Mutant Phenotype Assigned on 2007-03-08	Celenza JL and Carlson M  (1989) Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein. Mol Cell Biol 9(11):5034-44	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
peroxisome organization	IMP: Inferred from Mutant Phenotype Assigned on 2002-08-06	Simon M, et al.  (1992) Control of peroxisome proliferation in Saccharomyces cerevisiae by ADR1, SNF1 (CAT1, CCR1) and SNF4 (CAT3). Yeast 8(4):303-9	SGD
positive regulation of gluconeogenesis	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-12 IPI: Inferred from Physical Interaction with SGD:SNF4 Assigned on 2007-03-12	Celenza JL, et al.  (1989) Molecular analysis of the SNF4 gene of Saccharomyces cerevisiae: evidence for physical association of the SNF4 protein with the SNF1 protein kinase. Mol Cell Biol 9(11):5045-54	SGD
protein phosphorylation	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-12	McCartney RR and Schmidt MC  (2001) Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit. J Biol Chem 276(39):36460-6	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-12	Celenza JL and Carlson M  (1989) Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein. Mol Cell Biol 9(11):5034-44	SGD
	IGI: Inferred from Genetic Interaction with SGD:SNF1 Assigned on 2007-03-12 IMP: Inferred from Mutant Phenotype Assigned on 2007-03-12	Woods A, et al.  (1994) Yeast SNF1 is functionally related to mammalian AMP-activated protein kinase and regulates acetyl-CoA carboxylase in vivo. J Biol Chem 269(30):19509-15	SGD
regulation of transcription from RNA polymerase II promoter	IGI: Inferred from Genetic Interaction with SGD:SNF1 Assigned on 2007-03-12	Celenza JL and Carlson M  (1989) Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein. Mol Cell Biol 9(11):5034-44	SGD
replicative cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-09	Ashrafi K, et al.  (2000) Sip2p and its partner snf1p kinase affect aging in S. cerevisiae. Genes Dev 14(15):1872-85	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
AMP-activated protein kinase complex	IPI: Inferred from Physical Interaction with SGD:SIP1, SGD:GAL83, SGD:SIP2, SGD:SNF1 Assigned on 2007-03-09	Jiang R and Carlson M  (1997) The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex. Mol Cell Biol 17(4):2099-106	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-03-12	Nath N, et al.  (2002) Purification and characterization of Snf1 kinase complexes containing a defined Beta subunit composition. J Biol Chem 277(52):50403-8	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-03-08 IPI: Inferred from Physical Interaction with SGD:SNF1 Assigned on 2007-03-08	Celenza JL, et al.  (1989) Molecular analysis of the SNF4 gene of Saccharomyces cerevisiae: evidence for physical association of the SNF4 protein with the SNF1 protein kinase. Mol Cell Biol 9(11):5045-54	SGD
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2007-02-09	Sarma NJ, et al.  (2007) Glucose-responsive regulators of gene expression in Saccharomyces cerevisiae function at the nuclear periphery via a reverse recruitment mechanism. Genetics 175(3):1127-35	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-08-06	Celenza JL, et al.  (1989) Molecular analysis of the SNF4 gene of Saccharomyces cerevisiae: evidence for physical association of the SNF4 protein with the SNF1 protein kinase. Mol Cell Biol 9(11):5045-54	SGD
nuclear envelope lumen	IDA: Inferred from Direct Assay Assigned on 2007-02-09	Sarma NJ, et al.  (2007) Glucose-responsive regulators of gene expression in Saccharomyces cerevisiae function at the nuclear periphery via a reverse recruitment mechanism. Genetics 175(3):1127-35	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2007-02-09	Sarma NJ, et al.  (2007) Glucose-responsive regulators of gene expression in Saccharomyces cerevisiae function at the nuclear periphery via a reverse recruitment mechanism. Genetics 175(3):1127-35	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-08-06	Celenza JL, et al.  (1989) Molecular analysis of the SNF4 gene of Saccharomyces cerevisiae: evidence for physical association of the SNF4 protein with the SNF1 protein kinase. Mol Cell Biol 9(11):5045-54	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2003-04-25	Lin SS, et al.  (2003) Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing. J Biol Chem 278(15):13390-7	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SNF4

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR013785 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
carbohydrate metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0119 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).