This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-12-15    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin binding	IDA: Inferred from Direct Assay Assigned on 2009-08-18	Li XY, et al.  (2000) Distinct classes of yeast promoters revealed by differential TAF recruitment. Science 288(5469):1242-4	SGD
protein complex scaffold	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-18	Moqtaderi Z, et al.  (1998) The histone H3-like TAF is broadly required for transcription in yeast. Mol Cell 2(5):675-82	SGD
RNA polymerase II activating transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2011-12-15 IPI: Inferred from Physical Interaction with SGD:GAL4 Assigned on 2011-12-15	Klein J, et al.  (2003) Use of a genetically introduced cross-linker to identify interaction sites of acidic activators within native transcription factor IID and SAGA. J Biol Chem 278(9):6779-86	SGD
contributes_to RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly	IC: Inferred By Curator from RNA polymerase II transcriptional preinitiation complex assembly, transcription factor TFIID complex Assigned on 2011-12-13	GO Annotation Working Group  (2011) Manual annotations that require more than one source of functional data to support the assignment of the associated GO term.	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin modification	IDA: Inferred from Direct Assay Assigned on 2009-09-03	Grant PA, et al.  (1998) A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation. Cell 94(1):45-53	SGD
histone acetylation	IDA: Inferred from Direct Assay Assigned on 2009-09-03	Grant PA, et al.  (1998) A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation. Cell 94(1):45-53	SGD
RNA polymerase II transcriptional preinitiation complex assembly	IMP: Inferred from Mutant Phenotype Assigned on 2008-07-24	Shen WC, et al.  (2003) Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly. EMBO J 22(13):3395-402	SGD
transcription from RNA polymerase II promoter	IDA: Inferred from Direct Assay Assigned on 2009-08-18	Sanders SL, et al.  (2002) Molecular characterization of Saccharomyces cerevisiae TFIID. Mol Cell Biol 22(16):6000-13	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-08-18	Auty R, et al.  (2004) Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem 279(48):49973-81	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
SAGA complex	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Grant PA, et al.  (1998) A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation. Cell 94(1):45-53	SGD
SLIK (SAGA-like) complex	IDA: Inferred from Direct Assay Assigned on 2009-09-03	Pray-Grant MG, et al.  (2002) The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway. Mol Cell Biol 22(24):8774-86	SGD
transcription factor TFIID complex	IDA: Inferred from Direct Assay Assigned on 2008-07-24	Sanders SL and Weil PA  (2000) Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex. J Biol Chem 275(18):13895-900	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-12-15	Auty R, et al.  (2004) Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem 279(48):49973-81	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for TAF6

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein heterodimerization activity	IEA: Inferred from Electronic Annotation with EBI:IPR009072 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-dependent transcription, initiation	IEA: Inferred from Electronic Annotation with EBI:IPR004823 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of sequence-specific DNA binding transcription factor activity	IEA: Inferred from Electronic Annotation with EBI:IPR011442 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR011442, EBI:IPR004823 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).