This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-28    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA binding	IDA: Inferred from Direct Assay Assigned on 2008-07-02	Miller MT, et al.  (2008) Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p. Nat Struct Mol Biol 15(4):397-402	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-09-24	Olivas W and Parker R  (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
negative regulation of chromatin silencing involved in replicative cell aging	IGI: Inferred from Genetic Interaction Assigned on 2003-08-14 IMP: Inferred from Mutant Phenotype Assigned on 2003-08-14	Kennedy BK, et al.  (1997) Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell 89(3):381-91	SGD
negative regulation of translation	IDA: Inferred from Direct Assay Assigned on 2010-05-06	Chritton JJ and Wickens M  (2010) Translational repression by PUF proteins in vitro. RNA 16(6):1217-25	SGD
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IGI: Inferred from Genetic Interaction Assigned on 2002-09-24	Olivas W and Parker R  (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11	SGD
protein localization	IMP: Inferred from Mutant Phenotype Assigned on 2005-07-28	Kennedy BK, et al.  (1997) Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell 89(3):381-91	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function | Cellular Component

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA binding	IDA: Inferred from Direct Assay Assigned on 2006-08-17	Gerber AP, et al.  (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79	SGD

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
RNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR001313 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).