This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2012-05-11    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2012-05-11	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum organization	IGI: Inferred from Genetic Interaction with EBI:Q99287 Assigned on 2011-01-26	Hu J, et al.  (2009) A class of dynamin-like GTPases involved in the generation of the tubular ER network. Cell 138(3):549-61	UniProtKB
endoplasmic reticulum tubular network maintenance	IGI: Inferred from Genetic Interaction with SGD:YOP1, SGD:RTN2 Assigned on 2012-05-09	West M, et al.  (2011) A 3D analysis of yeast ER structure reveals how ER domains are organized by membrane curvature. J Cell Biol 193(2):333-46	SGD
endoplasmic reticulum tubular network organization	IGI: Inferred from Genetic Interaction with SGD:YOP1, SGD:RTN2 Assigned on 2012-05-09 IMP: Inferred from Mutant Phenotype Assigned on 2011-01-26	Voeltz GK, et al.  (2006) A class of membrane proteins shaping the tubular endoplasmic reticulum. Cell 124(3):573-86	SGD, UniProtKB
	IGI: Inferred from Genetic Interaction with SGD:YOP1, SGD:RTN2 Assigned on 2012-05-11	Shibata Y, et al.  (2008) The reticulon and dp1/yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum. J Biol Chem 283(27):18892-904	SGD
	IGI: Inferred from Genetic Interaction with SGD:YOP1, SGD:RTN2 Assigned on 2012-05-11	Hu J, et al.  (2008) Membrane proteins of the endoplasmic reticulum induce high-curvature tubules. Science 319(5867):1247-50	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-01-26	De Craene JO, et al.  (2006) Rtn1p is involved in structuring the cortical endoplasmic reticulum. Mol Biol Cell 17(7):3009-20	UniProtKB
	IGI: Inferred from Genetic Interaction with SGD:SEY1 Assigned on 2012-05-11 IPI: Inferred from Physical Interaction with SGD:SEY1 Assigned on 2012-05-11	Hu J, et al.  (2009) A class of dynamin-like GTPases involved in the generation of the tubular ER network. Cell 138(3):549-61	SGD
nuclear pore complex assembly	IGI: Inferred from Genetic Interaction with SGD:NUP116, SGD:POM152, SGD:NUP188, SGD:NUP145, SGD:YOP1, SGD:NEM1, SGD:GLE2, SGD:NUP85, SGD:NUP82, SGD:SPO7, SGD:NUP120, SGD:POM34 Assigned on 2012-05-11	Dawson TR, et al.  (2009) ER membrane-bending proteins are necessary for de novo nuclear pore formation. J Cell Biol 184(5):659-75	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cortical endoplasmic reticulum	IDA: Inferred from Direct Assay Assigned on 2008-10-13	De Craene JO, et al.  (2006) Rtn1p is involved in structuring the cortical endoplasmic reticulum. Mol Biol Cell 17(7):3009-20	SGD
endoplasmic reticulum	IDA: Inferred from Direct Assay Assigned on 2006-04-26	Geng J, et al.  (2005) Saccharomyces cerevisiae Rab-GDI displacement factor ortholog Yip3p forms distinct complexes with the Ypt1 Rab GTPase and the reticulon Rtn1p. Eukaryot Cell 4(7):1166-74	SGD
endoplasmic reticulum tubular network	IDA: Inferred from Direct Assay Assigned on 2012-05-09	Voeltz GK, et al.  (2006) A class of membrane proteins shaping the tubular endoplasmic reticulum. Cell 124(3):573-86	SGD
Golgi apparatus	IDA: Inferred from Direct Assay Assigned on 2006-04-26	Geng J, et al.  (2005) Saccharomyces cerevisiae Rab-GDI displacement factor ortholog Yip3p forms distinct complexes with the Ypt1 Rab GTPase and the reticulon Rtn1p. Eukaryot Cell 4(7):1166-74	SGD
integral to endoplasmic reticulum membrane	IDA: Inferred from Direct Assay Assigned on 2008-10-13	De Craene JO, et al.  (2006) Rtn1p is involved in structuring the cortical endoplasmic reticulum. Mol Biol Cell 17(7):3009-20	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-28	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum	IEA: Inferred from Electronic Annotation with EBI:IPR003388 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0256 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
endoplasmic reticulum membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0097 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).