This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-10-04    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
RNA polymerase II activating transcription factor binding	IPI: Inferred from Physical Interaction with SGD:GCN5, SGD:ADA2 Assigned on 2011-10-04	Chiang YC, et al.  (1996) ADR1 activation domains contact the histone acetyltransferase GCN5 and the core transcriptional factor TFIIB. J Biol Chem 271(50):32359-65	SGD
RNA polymerase II core promoter proximal region sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Thukral SK, et al.  (1991) Two monomers of yeast transcription factor ADR1 bind a palindromic sequence symmetrically to activate ADH2 expression. Mol Cell Biol 11(3):1566-77	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Grauslund M, et al.  (1999) Expression of GUT1, which encodes glycerol kinase in Saccharomyces cerevisiae, is controlled by the positive regulators Adr1p, Ino2p and Ino4p and the negative regulator Opi1p in a carbon source-dependent fashion. Nucleic Acids Res 27(22):4391-8	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Schmiedeskamp M, et al.  (1997) NMR chemical shift perturbation mapping of DNA binding by a zinc-finger domain from the yeast transcription factor ADR1. Protein Sci 6(9):1835-48	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Camier S, et al.  (1992) A mutation outside the two zinc fingers of ADR1 can suppress defects in either finger. Mol Cell Biol 12(12):5758-67	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Rottensteiner H, et al.  (2003) Saccharomyces cerevisiae PIP2 mediating oleic acid induction and peroxisome proliferation is regulated by Adr1p and Pip2p-Oaf1p. J Biol Chem 278(30):27605-11	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Simon M, et al.  (1991) The Saccharomyces cerevisiae ADR1 gene is a positive regulator of transcription of genes encoding peroxisomal proteins. Mol Cell Biol 11(2):699-704	SGD
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Grauslund M, et al.  (1999) Expression of GUT1, which encodes glycerol kinase in Saccharomyces cerevisiae, is controlled by the positive regulators Adr1p, Ino2p and Ino4p and the negative regulator Opi1p in a carbon source-dependent fashion. Nucleic Acids Res 27(22):4391-8	SGD
RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription	IPI: Inferred from Physical Interaction with SGD:GCN5, SGD:ADA2 Assigned on 2011-10-04	Chiang YC, et al.  (1996) ADR1 activation domains contact the histone acetyltransferase GCN5 and the core transcriptional factor TFIIB. J Biol Chem 271(50):32359-65	SGD
TFIIB-class transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Chiang YC, et al.  (1996) ADR1 activation domains contact the histone acetyltransferase GCN5 and the core transcriptional factor TFIIB. J Biol Chem 271(50):32359-65	SGD
TFIID-class transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Komarnitsky PB, et al.  (1998) ADR1-mediated transcriptional activation requires the presence of an intact TFIID complex. Mol Cell Biol 18(10):5861-7	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular response to oleic acid	IPI: Inferred from Physical Interaction Assigned on 2011-10-25	Smith JJ, et al.  (2007) Transcriptional responses to fatty acid are coordinated by combinatorial control. Mol Syst Biol 3():115	SGD
chromatin organization	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Di Mauro E, et al.  (2000) Two distinct nucleosome alterations characterize chromatin remodeling at the Saccharomyces cerevisiae ADH2 promoter. J Biol Chem 275(11):7612-8	SGD
negative regulation of chromatin silencing	IDA: Inferred from Direct Assay Assigned on 2011-10-04	Yu Q, et al.  (2003) Rap1p and other transcriptional regulators can function in defining distinct domains of gene expression. Nucleic Acids Res 31(4):1224-33	SGD
peroxisome organization	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-04	Simon M, et al.  (1992) Control of peroxisome proliferation in Saccharomyces cerevisiae by ADR1, SNF1 (CAT1, CCR1) and SNF4 (CAT3). Yeast 8(4):303-9	SGD
positive regulation of ethanol catabolic process by positive regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2012-02-09	Sloan JS, et al.  (1999) Post-translational regulation of Adr1 activity is mediated by its DNA binding domain. J Biol Chem 274(53):37575-82	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-02-09	Donoviel MS, et al.  (1995) Synergistic activation of ADH2 expression is sensitive to upstream activation sequence 2 (UAS2) orientation, copy number and UAS1-UAS2 helical phasing. Mol Cell Biol 15(6):3442-9	SGD
positive regulation of fatty acid beta-oxidation by positive regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-01	Simon M, et al.  (1991) The Saccharomyces cerevisiae ADR1 gene is a positive regulator of transcription of genes encoding peroxisomal proteins. Mol Cell Biol 11(2):699-704	SGD
positive regulation of peroxisome organization by positive regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2012-02-09	Simon MM, et al.  (1995) A C-terminal region of the Saccharomyces cerevisiae transcription factor ADR1 plays an important role in the regulation of peroxisome proliferation by fatty acids. Mol Gen Genet 249(3):289-96	SGD
positive regulation of transcription from RNA polymerase II promoter by oleic acid	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-07	Gurvitz A, et al.  (2001) Saccharomyces cerevisiae Adr1p governs fatty acid beta-oxidation and peroxisome proliferation by regulating POX1 and PEX11. J Biol Chem 276(34):31825-30	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-07	Simon M, et al.  (1992) Control of peroxisome proliferation in Saccharomyces cerevisiae by ADR1, SNF1 (CAT1, CCR1) and SNF4 (CAT3). Yeast 8(4):303-9	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-07	Rottensteiner H, et al.  (2003) Saccharomyces cerevisiae PIP2 mediating oleic acid induction and peroxisome proliferation is regulated by Adr1p and Pip2p-Oaf1p. J Biol Chem 278(30):27605-11	SGD
positive regulation of transcription from RNA polymerase II promoter in response to ethanol	IMP: Inferred from Mutant Phenotype Assigned on 2012-01-17	Pavlik P, et al.  (1993) The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization. Curr Genet 24(1-2):21-5	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-01-17	Simon M, et al.  (1992) Control of peroxisome proliferation in Saccharomyces cerevisiae by ADR1, SNF1 (CAT1, CCR1) and SNF4 (CAT3). Yeast 8(4):303-9	SGD
regulation of transcription by chromatin organization	IGI: Inferred from Genetic Interaction with SGD:GCN5, SGD:ADA2 Assigned on 2011-10-04 IPI: Inferred from Physical Interaction with SGD:GCN5, SGD:ADA2 Assigned on 2011-10-04	Chiang YC, et al.  (1996) ADR1 activation domains contact the histone acetyltransferase GCN5 and the core transcriptional factor TFIIB. J Biol Chem 271(50):32359-65	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-04	Agricola E, et al.  (2004) Common chromatin architecture, common chromatin remodeling, and common transcription kinetics of Adr1-dependent genes in Saccharomyces cerevisiae. Biochemistry 43(27):8878-84	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-07-26	Sloan JS, et al.  (1999) Post-translational regulation of Adr1 activity is mediated by its DNA binding domain. J Biol Chem 274(53):37575-82	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function | Cellular Component

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2009-02-05	Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87	SGD

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleic acid binding	IEA: Inferred from Electronic Annotation with EBI:IPR013087 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
zinc ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR015880, EBI:IPR007087 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
intracellular	IEA: Inferred from Electronic Annotation with EBI:IPR015880 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).