This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-01-22    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
inositol tetrakisphosphate 3-kinase activity	IDA: Inferred from Direct Assay Assigned on 2008-01-22	Saiardi A, et al.  (1999) Synthesis of diphosphoinositol pentakisphosphate by a newly identified family of higher inositol polyphosphate kinases. Curr Biol 9(22):1323-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-01-22	Odom AR, et al.  (2000) A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control. Science 287(5460):2026-9	SGD
inositol tetrakisphosphate 6-kinase activity	IDA: Inferred from Direct Assay Assigned on 2008-01-22	Zhang T, et al.  (2001) The transcriptional regulator, Arg82, is a hybrid kinase with both monophosphoinositol and diphosphoinositol polyphosphate synthase activity. FEBS Lett 494(3):208-12	SGD
inositol-1,3,4,5,6-pentakisphosphate kinase activity	IDA: Inferred from Direct Assay Assigned on 2008-01-22	Zhang T, et al.  (2001) The transcriptional regulator, Arg82, is a hybrid kinase with both monophosphoinositol and diphosphoinositol polyphosphate synthase activity. FEBS Lett 494(3):208-12	SGD
inositol-1,4,5-trisphosphate 3-kinase activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-19	Saiardi A, et al.  (2000) Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae. FEBS Lett 468(1):28-32	SGD
inositol-1,4,5-trisphosphate 6-kinase activity	IDA: Inferred from Direct Assay Assigned on 2008-01-22	Saiardi A, et al.  (1999) Synthesis of diphosphoinositol pentakisphosphate by a newly identified family of higher inositol polyphosphate kinases. Curr Biol 9(22):1323-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-01-22	Odom AR, et al.  (2000) A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control. Science 287(5460):2026-9	SGD
phosphatidylinositol 3-kinase activity	IDA: Inferred from Direct Assay Assigned on 2006-08-31	Resnick AC, et al.  (2005) Inositol polyphosphate multikinase is a nuclear PI3-kinase with transcriptional regulatory activity. Proc Natl Acad Sci U S A 102(36):12783-8	SGD
protein binding, bridging	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-19 IPI: Inferred from Physical Interaction with SGD:ARG80, SGD:MCM1 Assigned on 2007-12-19	El Bakkoury M, et al.  (2000) Recruitment of the yeast MADS-box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability. Mol Microbiol 35(1):15-31	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
inositol phosphate biosynthetic process	IDA: Inferred from Direct Assay Assigned on 2007-12-19	Saiardi A, et al.  (1999) Synthesis of diphosphoinositol pentakisphosphate by a newly identified family of higher inositol polyphosphate kinases. Curr Biol 9(22):1323-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-12-19	Zhang T, et al.  (2001) The transcriptional regulator, Arg82, is a hybrid kinase with both monophosphoinositol and diphosphoinositol polyphosphate synthase activity. FEBS Lett 494(3):208-12	SGD
negative regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-19	El Alami M, et al.  (2003) Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast. Mol Microbiol 49(2):457-68	SGD
phosphatidylinositol phosphorylation	IDA: Inferred from Direct Assay Assigned on 2007-12-19	Resnick AC, et al.  (2005) Inositol polyphosphate multikinase is a nuclear PI3-kinase with transcriptional regulatory activity. Proc Natl Acad Sci U S A 102(36):12783-8	SGD
positive regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-19	El Alami M, et al.  (2003) Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast. Mol Microbiol 49(2):457-68	SGD
protein stabilization	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-19	El Bakkoury M, et al.  (2000) Recruitment of the yeast MADS-box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability. Mol Microbiol 35(1):15-31	SGD
regulation of arginine metabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-19	Dubois E, et al.  (2000) Inositol polyphosphate kinase activity of Arg82/ArgRIII is not required for the regulation of the arginine metabolism in yeast. FEBS Lett 486(3):300-4	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-19	Dubois E and Messenguy F  (1994) Pleiotropic function of ArgRIIIp (Arg82p), one of the regulators of arginine metabolism in Saccharomyces cerevisiae. Role in expression of cell-type-specific genes. Mol Gen Genet 243(3):315-24	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-19	Qiu HF, et al.  (1990) Functional analysis of ARGRI and ARGRIII regulatory proteins involved in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Gen Genet 222(2-3):192-200	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-07-29	Bercy J, et al.  (1987) Regulation of arginine metabolism in Saccharomyces cerevisiae: expression of the three ARGR regulatory genes and cellular localization of their products. Gene 55(2-3):277-85	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-08-31	Odom AR, et al.  (2000) A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control. Science 287(5460):2026-9	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for ARG82

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
inositol-1,4,5-trisphosphate 3-kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR005522 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
inositol-1,4,5-trisphosphate 6-kinase activity	IEA: Inferred from Electronic Annotation with IUBMB:2.7.1.151 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
kinase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
arginine metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0056 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphorylation	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).