This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2013-02-28    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2011-01-06	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum inheritance	IMP: Inferred from Mutant Phenotype Assigned on 2012-07-24	Wiederkehr A, et al.  (2004) Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol 167(5):875-87	SGD
exocyst assembly	IMP: Inferred from Mutant Phenotype Assigned on 2012-08-31	Wiederkehr A, et al.  (2004) Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol 167(5):875-87	SGD
exocytosis	IGI: Inferred from Genetic Interaction with SGD:SEC4, SGD:SEC1 Assigned on 2012-07-25	Wiederkehr A, et al.  (2004) Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol 167(5):875-87	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-07-12	Novick P, et al.  (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15	SGD
Golgi to plasma membrane transport	IGI: Inferred from Genetic Interaction with SGD:SEC7 Assigned on 2012-07-19 IMP: Inferred from Mutant Phenotype Assigned on 2012-07-19	Novick P, et al.  (1981) Order of events in the yeast secretory pathway. Cell 25(2):461-9	SGD
vesicle tethering involved in exocytosis	IC: Inferred By Curator from exocyst Assigned on 2013-01-11	TerBush DR, et al.  (1996) The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15(23):6483-94	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2005-07-28	Zhang X, et al.  (2001) Cdc42 interacts with the exocyst and regulates polarized secretion. J Biol Chem 276(50):46745-50	SGD
cellular bud tip	IDA: Inferred from Direct Assay Assigned on 2005-07-28	Zhang X, et al.  (2001) Cdc42 interacts with the exocyst and regulates polarized secretion. J Biol Chem 276(50):46745-50	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-09-07	He B, et al.  (2007) Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. EMBO J 26(18):4053-65	SGD
exocyst	IDA: Inferred from Direct Assay Assigned on 2011-01-05	TerBush DR, et al.  (1996) The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15(23):6483-94	SGD
incipient cellular bud site	IDA: Inferred from Direct Assay Assigned on 2005-07-28	Zhang X, et al.  (2001) Cdc42 interacts with the exocyst and regulates polarized secretion. J Biol Chem 276(50):46745-50	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mating projection tip	IDA: Inferred from Direct Assay Assigned on 2008-12-19	Narayanaswamy R, et al.  (2009) Systematic Definition of Protein Constituents along the Major Polarization Axis Reveals an Adaptive Reuse of the Polarization Machinery in Pheromone-Treated Budding Yeast. J Proteome Res 8(1):6-19	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
exocytosis	IEA: Inferred from Electronic Annotation with EBI:KW-0268 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).