This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-06-03    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
dihydrolipoyllysine-residue succinyltransferase activity	ISA: Inferred from Sequence Alignment with EBI:P0AFG6 Assigned on 2010-06-03	Repetto B and Tzagoloff A  (1990) Structure and regulation of KGD2, the structural gene for yeast dihydrolipoyl transsuccinylase. Mol Cell Biol 10(8):4221-32	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
2-oxoglutarate metabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-03	Repetto B and Tzagoloff A  (1990) Structure and regulation of KGD2, the structural gene for yeast dihydrolipoyl transsuccinylase. Mol Cell Biol 10(8):4221-32	SGD
mitochondrial genome maintenance	IGI: Inferred from Genetic Interaction with SGD:ABF2 Assigned on 2010-06-03	Kaufman BA, et al.  (2000) In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins. Proc Natl Acad Sci U S A 97(14):7772-7	SGD
tricarboxylic acid cycle	IC: Inferred By Curator from mitochondrial oxoglutarate dehydrogenase complex Assigned on 2010-06-03	Repetto B and Tzagoloff A  (1991) In vivo assembly of yeast mitochondrial alpha-ketoglutarate dehydrogenase complex. Mol Cell Biol 11(8):3931-9	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial nucleoid	IDA: Inferred from Direct Assay Assigned on 2005-04-01	Kaufman BA, et al.  (2000) In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins. Proc Natl Acad Sci U S A 97(14):7772-7	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-12-18	Sato H, et al.  (2002) Identification of the YMN-1 antigen protein and biochemical analyses of protein components in the mitochondrial nucleoid fraction of the yeast Saccharomyces cerevisiae. Protoplasma 219(1-2):51-8	SGD
mitochondrial oxoglutarate dehydrogenase complex	IDA: Inferred from Direct Assay Assigned on 2004-12-07	Repetto B and Tzagoloff A  (1991) In vivo assembly of yeast mitochondrial alpha-ketoglutarate dehydrogenase complex. Mol Cell Biol 11(8):3931-9	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
dihydrolipoyllysine-residue succinyltransferase activity	IEA: Inferred from Electronic Annotation with EBI:IPR006255 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:2.3.1.61 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring acyl groups	IEA: Inferred from Electronic Annotation with EBI:IPR001078 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0012 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
L-lysine catabolic process to acetyl-CoA via saccharopine	IEA: Inferred from Electronic Annotation with UniPathway:UPA00868 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR001078 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
tricarboxylic acid cycle	IEA: Inferred from Electronic Annotation with EBI:IPR006255 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0816 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:SL-0173 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxoglutarate dehydrogenase complex	IEA: Inferred from Electronic Annotation with EBI:IPR006255 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).