This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2005-04-27    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein tag	ISS: Inferred from Sequence or structural Similarity with SGD:SMT3, SGD:UBI4 Assigned on 2005-10-18 IDA: Inferred from Direct Assay Assigned on 2005-04-27	Liakopoulos D, et al.  (1998) A novel protein modification pathway related to the ubiquitin system. EMBO J 17(8):2208-14	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-04-27	Lammer D, et al.  (1998) Modification of yeast Cdc53p by the ubiquitin-related protein rub1p affects function of the SCFCdc4 complex. Genes Dev 12(7):914-26	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein neddylation	IDA: Inferred from Direct Assay Assigned on 2005-04-27	Liakopoulos D, et al.  (1998) A novel protein modification pathway related to the ubiquitin system. EMBO J 17(8):2208-14	SGD
	IGI: Inferred from Genetic Interaction with SGD:CDC34, SGD:CDC53 Assigned on 2005-04-27 IDA: Inferred from Direct Assay Assigned on 2005-04-27	Lammer D, et al.  (1998) Modification of yeast Cdc53p by the ubiquitin-related protein rub1p affects function of the SCFCdc4 complex. Genes Dev 12(7):914-26	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

There are no Computational annotations for RUB1

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).