This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-05-11    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
bilirubin transmembrane transporter activity	IGI: Inferred from Genetic Interaction Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Petrovic S, et al.  (2000) The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast 16(6):561-71	SGD
glutathione S-conjugate-exporting ATPase activity	IDA: Inferred from Direct Assay Assigned on 2004-05-11	Gomes DS, et al.  (2002) Regulation of cadmium uptake by Saccharomyces cerevisiae. Biochim Biophys Acta 1573(1):21-5	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-05-11	Gueldry O, et al.  (2003) Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur J Biochem 270(11):2486-96	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-05-11	Ghosh M, et al.  (1999) Pathways of As(III) detoxification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96(9):5001-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-05-11	Ishikawa T, et al.  (1997) The GS-X pump in plant, yeast, and animal cells: structure, function, and gene expression. Biosci Rep 17(2):189-207	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
bilirubin transport	IGI: Inferred from Genetic Interaction Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Petrovic S, et al.  (2000) The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast 16(6):561-71	SGD
response to metal ion	IDA: Inferred from Direct Assay Assigned on 2004-05-11	Gueldry O, et al.  (2003) Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur J Biochem 270(11):2486-96	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-05-11	Ghosh M, et al.  (1999) Pathways of As(III) detoxification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96(9):5001-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-05-11	Ishikawa T, et al.  (1997) The GS-X pump in plant, yeast, and animal cells: structure, function, and gene expression. Biosci Rep 17(2):189-207	SGD
transmembrane transport	IMP: Inferred from Mutant Phenotype Assigned on 2009-01-14	Petrovic S, et al.  (2000) The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast 16(6):561-71	SGD
vacuole fusion, non-autophagic	IMP: Inferred from Mutant Phenotype Assigned on 2013-05-15	Sasser TL, et al.  (2013) The Yeast ABC Transporter Ycf1p Enhances the Recruitment of the Soluble SNARE Vam7p to Vacuoles for Efficient Membrane Fusion. J Biol Chem ()	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type vacuole membrane	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Tommasini R, et al.  (1996) The human multidrug resistance-associated protein functionally complements the yeast cadmium resistance factor 1. Proc Natl Acad Sci U S A 93(13):6743-8	SGD
	IDA: Inferred from Direct Assay Assigned on 2013-05-15	Sasser TL, et al.  (2013) The Yeast ABC Transporter Ycf1p Enhances the Recruitment of the Soluble SNARE Vam7p to Vacuoles for Efficient Membrane Fusion. J Biol Chem ()	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR003439, EBI:IPR011527, EBI:IPR001140, EBI:IPR017871 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ATPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003439, EBI:IPR017871 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATPase activity, coupled to transmembrane movement of substances	IEA: Inferred from Electronic Annotation with EBI:IPR011527, EBI:IPR001140 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleoside-triphosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003593 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR003593 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR003439, EBI:IPR017871 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC
response to cadmium ion	IEA: Inferred from Electronic Annotation with EBI:KW-0105 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transmembrane transport	IEA: Inferred from Electronic Annotation with EBI:IPR011527, EBI:IPR001140 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transport	IEA: Inferred from Electronic Annotation with EBI:IPR011527, EBI:IPR001140 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:IPR011527, EBI:IPR001140 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuolar membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0271 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuole	IEA: Inferred from Electronic Annotation with EBI:KW-0926 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).