This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-12-16    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
rDNA spacer replication fork barrier binding	IDA: Inferred from Direct Assay Assigned on 2009-04-27	Kobayashi T  (2003) The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Mol Cell Biol 23(24):9178-88	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin silencing at rDNA	IDA: Inferred from Direct Assay Assigned on 2003-10-10	Huang J and Moazed D  (2003) Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing. Genes Dev 17(17):2162-76	SGD
DNA recombination	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Kobayashi T and Horiuchi T  (1996) A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities. Genes Cells 1(5):465-74	SGD
extrachromosomal circular DNA accumulation involved in cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Defossez PA, et al.  (1999) Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell 3(4):447-55	SGD
negative regulation of DNA replication	IDA: Inferred from Direct Assay Assigned on 2003-12-16	Kobayashi T  (2003) The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Mol Cell Biol 23(24):9178-88	SGD
protein localization to nucleolar rDNA repeats	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-27	Johzuka K and Horiuchi T  (2009) The cis element and factors required for condensin recruitment to chromosomes. Mol Cell 34(1):26-35	SGD
rDNA condensation	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-16	Waples WG, et al.  (2009) Putting the Brake on FEAR: Tof2 Promotes the Biphasic Release of Cdc14 Phosphatase during Mitotic Exit. Mol Biol Cell 20(1):245-55	SGD
replicative cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-14	Kaeberlein M, et al.  (2005) Genes determining yeast replicative life span in a long-lived genetic background. Mech Ageing Dev 126(4):491-504	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Defossez PA, et al.  (1999) Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell 3(4):447-55	SGD
rDNA heterochromatin	IDA: Inferred from Direct Assay Assigned on 2012-03-15	Ha CW, et al.  (2012) Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae. Nucleic Acids Res 40(11):4892-903	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for FOB1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

There are no Computational annotations for FOB1

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).