This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-01-15    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2002-07-16	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
CVT pathway	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-21	Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-30	Nazarko TY, et al.  (2005) Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae. Autophagy 1(1):37-45	SGD
ER to Golgi vesicle-mediated transport	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-19	Sacher M, et al.  (2001) TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport. Mol Cell 7(2):433-42	SGD
	IGI: Inferred from Genetic Interaction with SGD:BET3 Assigned on 2010-11-19	Sacher M, et al.  (2000) Identification and characterization of five new subunits of TRAPP. Eur J Cell Biol 79(2):71-80	SGD
macroautophagy	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-21	Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-30	Nazarko TY, et al.  (2005) Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae. Autophagy 1(1):37-45	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-30	Meiling-Wesse K, et al.  (2005) Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway. J Biol Chem 280(39):33669-78	SGD
meiosis	IMP: Inferred from Mutant Phenotype Assigned on 2003-01-15	Kaytor MD and Livingston DM  (1995) GSG1, a yeast gene required for sporulation. Yeast 11(12):1147-55	SGD
peroxisome degradation	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-30	Nazarko TY, et al.  (2005) Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae. Autophagy 1(1):37-45	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-18	Meiling-Wesse K, et al.  (2005) Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway. J Biol Chem 280(39):33669-78	SGD
piecemeal microautophagy of nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-28	Krick R, et al.  (2008) Piecemeal microautophagy of the nucleus requires the core macroautophagy genes. Mol Biol Cell 19(10):4492-505	SGD
protein localization to pre-autophagosomal structure	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-30	Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6	SGD
vesicle organization	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-18	Meiling-Wesse K, et al.  (2005) Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway. J Biol Chem 280(39):33669-78	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
pre-autophagosomal structure	IDA: Inferred from Direct Assay Assigned on 2010-06-21	Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6	SGD
TRAPP complex	IDA: Inferred from Direct Assay Assigned on 2002-07-16	Sacher M, et al.  (2000) Identification and characterization of five new subunits of TRAPP. Eur J Cell Biol 79(2):71-80	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for TRS85

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagy	IEA: Inferred from Electronic Annotation with EBI:KW-0072 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
meiosis	IEA: Inferred from Electronic Annotation with EBI:KW-0469 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
sporulation resulting in formation of a cellular spore	IEA: Inferred from Electronic Annotation with EBI:KW-0749 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
pre-autophagosomal structure	IEA: Inferred from Electronic Annotation with EBI:SL-0220 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).