This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-03-01    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
disulfide oxidoreductase activity	IGI: Inferred from Genetic Interaction Assigned on 2002-04-16 ISS: Inferred from Sequence or structural Similarity Assigned on 2002-04-16 IMP: Inferred from Mutant Phenotype Assigned on 2002-04-16	Rodriguez-Manzaneque MT, et al.  (1999) Grx5 glutaredoxin plays a central role in protection against protein oxidative damage in Saccharomyces cerevisiae. Mol Cell Biol 19(12):8180-90	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
actin cytoskeleton organization	IMP: Inferred from Mutant Phenotype Assigned on 2011-03-18	Pujol-Carrion N and de la Torre-Ruiz MA  (2010) Glutaredoxins Grx4 and Grx3 of Saccharomyces cerevisiae Play a Role in Actin Dynamics through Their Trx Domains, Which Contributes to Oxidative Stress Resistance. Appl Environ Microbiol 76(23):7826-7835	SGD
cellular iron ion homeostasis	IGI: Inferred from Genetic Interaction with SGD:AFT1 Assigned on 2007-03-01 IPI: Inferred from Physical Interaction with SGD:AFT1 Assigned on 2007-03-01	Pujol-Carrion N, et al.  (2006) Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae. J Cell Sci 119(Pt 21):4554-64	SGD
cellular response to oxidative stress	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-01	Pujol-Carrion N, et al.  (2006) Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae. J Cell Sci 119(Pt 21):4554-64	SGD
	IGI: Inferred from Genetic Interaction Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Rodriguez-Manzaneque MT, et al.  (1999) Grx5 glutaredoxin plays a central role in protection against protein oxidative damage in Saccharomyces cerevisiae. Mol Cell Biol 19(12):8180-90	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2008-04-04	Kumanovics A, et al.  (2008) Identification of FRA1 and FRA2 as Genes Involved in Regulating the Yeast Iron Regulon in Response to Decreased Mitochondrial Iron-Sulfur Cluster Synthesis. J Biol Chem 283(16):10276-86	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2005-01-26	Molina MM, et al.  (2004) Nuclear monothiol glutaredoxins of Saccharomyces cerevisiae can function as mitochondrial glutaredoxins. J Biol Chem 279(50):51923-30	SGD
	IGI: Inferred from Genetic Interaction Assigned on 2007-03-01 IPI: Inferred from Physical Interaction Assigned on 2007-03-01	Pujol-Carrion N, et al.  (2006) Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae. J Cell Sci 119(Pt 21):4554-64	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for GRX3

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
2 iron, 2 sulfur cluster binding	IEA: Inferred from Electronic Annotation with EBI:KW-0001 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
electron carrier activity	IEA: Inferred from Electronic Annotation with EBI:IPR002109 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
iron-sulfur cluster binding	IEA: Inferred from Electronic Annotation with EBI:KW-0411 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein disulfide oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:IPR002109 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell redox homeostasis	IEA: Inferred from Electronic Annotation with EBI:IPR002109, EBI:IPR013766 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).