This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-09-22    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein homodimerization activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-04 IDA: Inferred from Direct Assay Assigned on 2007-06-04	Ptak C, et al.  (1994) Functional and physical characterization of the cell cycle ubiquitin-conjugating enzyme CDC34 (UBC3). Identification of a functional determinant within the tail that facilitates CDC34 self-association. J Biol Chem 269(42):26539-45	SGD
ubiquitin-protein ligase activity	ISS: Inferred from Sequence or structural Similarity with SGD:RAD6 Assigned on 2005-10-28 IMP: Inferred from Mutant Phenotype Assigned on 2005-10-28 IDA: Inferred from Direct Assay Assigned on 2007-03-01	Goebl MG, et al.  (1988) The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. Science 241(4871):1331-5	SGD
contributes_to ubiquitin-protein ligase activity	IDA: Inferred from Direct Assay Assigned on 2007-02-07	Feldman RM, et al.  (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-02-05	Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
G1/S transition of mitotic cell cycle	TAS: Traceable Author Statement Assigned on 2001-01-18	Hoyt MA  (1997) Eliminating all obstacles: regulated proteolysis in the eukaryotic cell cycle. Cell 91(2):149-51	SGD
G2/M transition of mitotic cell cycle	IGI: Inferred from Genetic Interaction with SGD:SIC1 Assigned on 2007-02-02	Jaquenoud M, et al.  (1998) The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex. EMBO J 17(18):5360-73	SGD
	IGI: Inferred from Genetic Interaction with SGD:SIC1 Assigned on 2007-02-02	Schwob E, et al.  (1994) The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae. Cell 79(2):233-44	SGD
protein autoubiquitination	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-02 IDA: Inferred from Direct Assay Assigned on 2007-03-02	Banerjee A, et al.  (1993) The bacterially expressed yeast CDC34 gene product can undergo autoubiquitination to form a multiubiquitin chain-linked protein. J Biol Chem 268(8):5668-75	SGD
protein polyubiquitination	IDA: Inferred from Direct Assay Assigned on 2005-10-28	Haas AL, et al.  (1991) Ubiquitin conjugation by the yeast RAD6 and CDC34 gene products. Comparison to their putative rabbit homologs, E2(20K) AND E2(32K). J Biol Chem 266(8):5104-12	SGD
protein ubiquitination involved in ubiquitin-dependent protein catabolic process	IDA: Inferred from Direct Assay Assigned on 2007-02-07	Feldman RM, et al.  (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-02-05	Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19	SGD
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IDA: Inferred from Direct Assay Assigned on 2005-04-04	Feldman RM, et al.  (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-02-05	Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-06-19	Blondel M, et al.  (2000) Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4. EMBO J 19(22):6085-97	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2007-03-28	Goebl MG, et al.  (1994) The Ubc3 (Cdc34) ubiquitin-conjugating enzyme is ubiquitinated and phosphorylated in vivo. Mol Cell Biol 14(5):3022-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-06-19	Blondel M, et al.  (2000) Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4. EMBO J 19(22):6085-97	SGD
SCF ubiquitin ligase complex	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-08	Jaquenoud M, et al.  (1998) The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex. EMBO J 17(18):5360-73	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-02-05 IPI: Inferred from Physical Interaction with SGD:CDC53 Assigned on 2007-03-01	Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for CDC34

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
acid-amino acid ligase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000608 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATP binding	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ligase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0436 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ubiquitin-protein ligase activity	IEA: Inferred from Electronic Annotation with IUBMB:6.3.2.19 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA replication	IEA: Inferred from Electronic Annotation with EBI:KW-0235 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein ubiquitination	IEA: Inferred from Electronic Annotation with UniPathway:UPA00143 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).