This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-07-31    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2008-07-31	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mitotic sister chromatid cohesion	IGI: Inferred from Genetic Interaction with SGD:ECO1 Assigned on 2008-07-31	Rolef Ben-Shahar T, et al.  (2008) Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion. Science 321(5888):563-6	SGD
negative regulation of maintenance of mitotic sister chromatid cohesion	IMP: Inferred from Mutant Phenotype Assigned on 2013-05-20	Lopez-Serra L, et al.  (2013) Budding yeast Wapl controls sister chromatid cohesion maintenance and chromosome condensation. Curr Biol 23(1):64-9	SGD
	IGI: Inferred from Genetic Interaction with SGD:ECO1 Assigned on 2013-05-21 IMP: Inferred from Mutant Phenotype Assigned on 2013-05-21	Chan KL, et al.  (2012) Cohesin's DNA exit gate is distinct from its entrance gate and is regulated by acetylation. Cell 150(5):961-74	SGD
regulation of chromosome condensation	IMP: Inferred from Mutant Phenotype Assigned on 2013-05-20	Lopez-Serra L, et al.  (2013) Budding yeast Wapl controls sister chromatid cohesion maintenance and chromosome condensation. Curr Biol 23(1):64-9	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
colocalizes_with nuclear cohesin complex	IDA: Inferred from Direct Assay Assigned on 2013-05-21	Chan KL, et al.  (2012) Cohesin's DNA exit gate is distinct from its entrance gate and is regulated by acetylation. Cell 150(5):961-74	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-12-17	Kulemzina I, et al.  (2012) Cohesin rings devoid of scc3 and pds5 maintain their stable association with the DNA. PLoS Genet 8(8):e1002856	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-07-31	Rolef Ben-Shahar T, et al.  (2008) Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion. Science 321(5888):563-6	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for RAD61

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).