This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-28    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
Rab GTPase activator activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-07-08 IMP: Inferred from Mutant Phenotype Assigned on 2002-07-08 IDA: Inferred from Direct Assay Assigned on 2002-07-08 IPI: Inferred from Physical Interaction Assigned on 2002-07-08	Albert S, et al.  (1999) Identification of the catalytic domains and their functionally critical arginine residues of two yeast GTPase-activating proteins specific for Ypt/Rab transport GTPases. EMBO J 18(19):5216-25	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of vacuole fusion, non-autophagic	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-01	Brett CL, et al.  (2008) Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase. J Cell Biol 182(6):1141-51	SGD
vesicle-mediated transport	IDA: Inferred from Direct Assay Assigned on 2002-07-08	Eitzen G, et al.  (2000) Sequential action of two GTPases to promote vacuole docking and fusion. EMBO J 19(24):6713-20	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTPase activator activity	IEA: Inferred from Electronic Annotation with EBI:KW-0343 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
Rab GTPase activator activity	IEA: Inferred from Electronic Annotation with EBI:IPR000195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
positive regulation of GTPase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0343 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	GOC
positive regulation of Rab GTPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC
regulation of Rab GTPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
intracellular	IEA: Inferred from Electronic Annotation with EBI:IPR000195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).