This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-10-07    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	IDA: Inferred from Direct Assay Assigned on 2011-10-07	Vrabioiu AM, et al.  (2004) The majority of the Saccharomyces cerevisiae septin complexes do not exchange guanine nucleotides. J Biol Chem 279(4):3111-8	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
barrier septum assembly	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-07	Iwase M, et al.  (2007) Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae. Genetics 177(1):215-29	SGD
cellular bud neck septin ring organization	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-07	Dobbelaere J, et al.  (2003) Phosphorylation-dependent regulation of septin dynamics during the cell cycle. Dev Cell 4(3):345-57	SGD
cytokinesis	IDA: Inferred from Direct Assay Assigned on 2011-10-07	Dobbelaere J, et al.  (2003) Phosphorylation-dependent regulation of septin dynamics during the cell cycle. Dev Cell 4(3):345-57	SGD
cytokinesis, actomyosin contractile ring assembly	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-07	Iwase M, et al.  (2007) Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae. Genetics 177(1):215-29	SGD
exit from mitosis	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-07	Castillon GA, et al.  (2003) Septins have a dual role in controlling mitotic exit in budding yeast. Curr Biol 13(8):654-8	SGD
G1 phase of mitotic cell cycle	IGI: Inferred from Genetic Interaction with SGD:CLN1, SGD:CLN2 Assigned on 2011-10-07	Egelhofer TA, et al.  (2008) The septins function in G1 pathways that influence the pattern of cell growth in budding yeast. PLoS ONE 3(4):e2022	SGD
septin ring assembly	IGI: Inferred from Genetic Interaction with SGD:CDC10 Assigned on 2011-10-07	Iwase M, et al.  (2007) Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae. Genetics 177(1):215-29	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck septin ring	IDA: Inferred from Direct Assay Assigned on 2011-10-07	Iwase M, et al.  (2007) Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae. Genetics 177(1):215-29	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mating projection tip	IDA: Inferred from Direct Assay Assigned on 2008-12-19	Narayanaswamy R, et al.  (2009) Systematic Definition of Protein Constituents along the Major Polarization Axis Reveals an Adaptive Reuse of the Polarization Machinery in Pheromone-Treated Budding Yeast. J Proteome Res 8(1):6-19	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	IEA: Inferred from Electronic Annotation with EBI:IPR000038 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0342 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:IPR000038 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IEA: Inferred from Electronic Annotation with EBI:SL-0029 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0162 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
septin complex	IEA: Inferred from Electronic Annotation with EBI:IPR016491 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).