This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-06-27    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
single-stranded telomeric DNA binding	IMP: Inferred from Mutant Phenotype Assigned on 2007-10-29 IDA: Inferred from Direct Assay Assigned on 2007-10-29	Lin YC, et al.  (2007) Genetic analysis reveals essential and non-essential amino acids within the telomeric DNA-binding interface of Cdc13p. Biochem J 403(2):289-95	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-10-29	Lin JJ and Zakian VA  (1996) The Saccharomyces CDC13 protein is a single-strand TG1-3 telomeric DNA-binding protein in vitro that affects telomere behavior in vivo. Proc Natl Acad Sci U S A 93(24):13760-5	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-10-29	Nugent CI, et al.  (1996) Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance. Science 274(5285):249-52	SGD
telomerase inhibitor activity	IDA: Inferred from Direct Assay Assigned on 2008-12-18	Zappulla DC, et al.  (2009) Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p. Nucleic Acids Res 37(2):354-67	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
negative regulation of telomerase activity	IDA: Inferred from Direct Assay Assigned on 2008-12-18	Zappulla DC, et al.  (2009) Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p. Nucleic Acids Res 37(2):354-67	SGD
regulation of telomere maintenance via telomerase	IGI: Inferred from Genetic Interaction with SGD:YKU80, SGD:STN1, SGD:YKU70 Assigned on 2007-06-27	Grandin N, et al.  (2000) Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitment. Mol Cell Biol 20(22):8397-408	SGD
telomere capping	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-27	Garvik B, et al.  (1995) Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint. Mol Cell Biol 15(11):6128-38	SGD
telomere maintenance	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-01	Meier B, et al.  (2001) New function of CDC13 in positive telomere length regulation. Mol Cell Biol 21(13):4233-45	SGD
telomere maintenance via telomerase	IGI: Inferred from Genetic Interaction with SGD:EST1, SGD:STN1 Assigned on 2007-06-27	Pennock E, et al.  (2001) Cdc13 delivers separate complexes to the telomere for end protection and replication. Cell 104(3):387-96	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-18	Lendvay TS, et al.  (1996) Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics 144(4):1399-412	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear telomere cap complex	IDA: Inferred from Direct Assay Assigned on 2003-04-03	Bourns BD, et al.  (1998) Sir proteins, Rif proteins, and Cdc13p bind Saccharomyces telomeres in vivo. Mol Cell Biol 18(9):5600-8	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for CDC13

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR011564 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
telomere maintenance	IEA: Inferred from Electronic Annotation with EBI:IPR011564 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome	IEA: Inferred from Electronic Annotation with EBI:KW-0158 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromosome, telomeric region	IEA: Inferred from Electronic Annotation with EBI:SL-0276 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0779 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear chromosome, telomeric region	IEA: Inferred from Electronic Annotation with EBI:IPR011564 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).