This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-07-02    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
centromere-specific nucleosome binding	IMP: Inferred from Mutant Phenotype Assigned on 2011-04-04 IPI: Inferred from Physical Interaction with SGD:CSE4 Assigned on 2011-04-04	Zhou Z, et al.  (2011) Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Nature 472(7342):234-7	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome segregation	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-02	Mizuguchi G, et al.  (2007) Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomes. Cell 129(6):1153-64	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-02	Camahort R, et al.  (2007) Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore. Mol Cell 26(6):853-65	SGD
G2/M transition of mitotic cell cycle	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-13	Stoler S, et al.  (2007) Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. Proc Natl Acad Sci U S A 104(25):10571-6	SGD
kinetochore assembly	IMP: Inferred from Mutant Phenotype Assigned on 2013-04-26	Camahort R, et al.  (2007) Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore. Mol Cell 26(6):853-65	SGD
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process	IDA: Inferred from Direct Assay Assigned on 2011-01-21	Hewawasam G, et al.  (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54	SGD
protein localization to chromosome, centromeric region	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-23	Camahort R, et al.  (2007) Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore. Mol Cell 26(6):853-65	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-23	Stoler S, et al.  (2007) Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. Proc Natl Acad Sci U S A 104(25):10571-6	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
condensed nuclear chromosome, centromeric region	IDA: Inferred from Direct Assay Assigned on 2007-07-02	Mizuguchi G, et al.  (2007) Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomes. Cell 129(6):1153-64	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-07-02	Camahort R, et al.  (2007) Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore. Mol Cell 26(6):853-65	SGD
	IPI: Inferred from Physical Interaction with SGD:CSE4 Assigned on 2007-07-02	Stoler S, et al.  (2007) Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. Proc Natl Acad Sci U S A 104(25):10571-6	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR018465 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).