This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-04-30    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein serine/threonine phosphatase activity	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-22	Sneddon AA, et al.  (1990) Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J 9(13):4339-46	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
actin filament organization	TAS: Traceable Author Statement Assigned on 2001-01-18	Wang Y and Burke DJ  (1997) Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol Cell Biol 17(2):620-6	SGD
budding cell bud growth	TAS: Traceable Author Statement Assigned on 2001-01-18	Wang Y and Burke DJ  (1997) Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol Cell Biol 17(2):620-6	SGD
G1/S transition of mitotic cell cycle	IGI: Inferred from Genetic Interaction with SGD:SIS2, SGD:SIT4 Assigned on 2003-02-17	Munoz I, et al.  (2003) Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae. Yeast 20(2):157-69	SGD
mitotic spindle assembly checkpoint	IPI: Inferred from Physical Interaction Assigned on 2001-01-18	Wang Y and Burke DJ  (1997) Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol Cell Biol 17(2):620-6	SGD
protein dephosphorylation	TAS: Traceable Author Statement Assigned on 2003-04-30	Wang Y and Burke DJ  (1997) Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol Cell Biol 17(2):620-6	SGD
regulation of translation	IPI: Inferred from Physical Interaction with SGD:TAP42 Assigned on 2009-09-22	Jiang Y and Broach JR  (1999) Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast. EMBO J 18(10):2782-92	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
protein phosphatase type 2A complex	IDA: Inferred from Direct Assay Assigned on 2007-08-02	Hombauer H, et al.  (2007) Generation of active protein phosphatase 2A is coupled to holoenzyme assembly. PLoS Biol 5(6):e155	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:IPR004843, EBI:IPR006186 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphoprotein phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0904 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with IUBMB:3.1.3.16 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).