This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2001-01-19    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
L-malate dehydrogenase activity	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-29	Minard KI and McAlister-Henn L  (1991) Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase. Mol Cell Biol 11(1):370-80	SGD
	TAS: Traceable Author Statement Assigned on 2005-04-29	McAlister-Henn L, et al.  (1995) Expression and function of a mislocalized form of peroxisomal malate dehydrogenase (MDH3) in yeast. J Biol Chem 270(36):21220-5	SGD
mRNA binding	IDA: Inferred from Direct Assay Assigned on 2011-03-30	Scherrer T, et al.  (2010) A Screen for RNA-Binding Proteins in Yeast Indicates Dual Functions for Many Enzymes. PLoS One 5(11):e15499	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
fatty acid beta-oxidation	TAS: Traceable Author Statement Assigned on 2001-01-18	McAlister-Henn L and Small WC  (1997) Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol 57:317-39	SGD
glyoxylate cycle	TAS: Traceable Author Statement Assigned on 2001-01-18	McAlister-Henn L and Small WC  (1997) Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol 57:317-39	SGD
malate metabolic process	TAS: Traceable Author Statement Assigned on 2001-01-18	McAlister-Henn L and Small WC  (1997) Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol 57:317-39	SGD
NADH regeneration	TAS: Traceable Author Statement Assigned on 2001-01-18	McAlister-Henn L and Small WC  (1997) Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol 57:317-39	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
peroxisomal matrix	IDA: Inferred from Direct Assay Assigned on 2013-03-13	Elgersma Y, et al.  (1996) Analysis of the carboxyl-terminal peroxisomal targeting signal 1 in a homologous context in Saccharomyces cerevisiae. J Biol Chem 271(42):26375-82	SGD
peroxisome	IDA: Inferred from Direct Assay Assigned on 2005-05-16	Steffan JS and McAlister-Henn L  (1992) Isolation and characterization of the yeast gene encoding the MDH3 isozyme of malate dehydrogenase. J Biol Chem 267(34):24708-15	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for MDH3

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR015955 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
L-malate dehydrogenase activity	IEA: Inferred from Electronic Annotation with EBI:IPR010097 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:1.1.1.37 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
malate dehydrogenase activity	IEA: Inferred from Electronic Annotation with EBI:IPR001252 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR016040 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:IPR001236 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	IEA: Inferred from Electronic Annotation with EBI:IPR022383, EBI:IPR001557, EBI:IPR015955 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
carbohydrate metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR015955 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
cellular carbohydrate metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR001557 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
glyoxylate cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0329 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
malate metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR010097, EBI:IPR001252 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
oxidation-reduction process	IEA: Inferred from Electronic Annotation with EBI:IPR022383, EBI:IPR010097, EBI:IPR001557, EBI:IPR001236, EBI:IPR001252, EBI:IPR015955 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
tricarboxylic acid cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0816 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
peroxisome	IEA: Inferred from Electronic Annotation with EBI:SL-0204 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0576 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).