This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2001-07-02    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
G-protein coupled receptor activity	IPI: Inferred from Physical Interaction with SGD:GPA2 Assigned on 2007-11-30	Yun CW, et al.  (1997) G-protein coupled receptor from yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun 240(2):287-92	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2001-07-02 IMP: Inferred from Mutant Phenotype Assigned on 2001-07-02 IPI: Inferred from Physical Interaction with SGD:GPA2 Assigned on 2007-11-30	Xue Y, et al.  (1998) GPR1 encodes a putative G protein-coupled receptor that associates with the Gpa2p Galpha subunit and functions in a Ras-independent pathway. EMBO J 17(7):1996-2007	SGD
glucose binding	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30	Lemaire K, et al.  (2004) Glucose and sucrose act as agonist and mannose as antagonist ligands of the G protein-coupled receptor Gpr1 in the yeast Saccharomyces cerevisiae. Mol Cell 16(2):293-9	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
detection of glucose	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30	Lemaire K, et al.  (2004) Glucose and sucrose act as agonist and mannose as antagonist ligands of the G protein-coupled receptor Gpr1 in the yeast Saccharomyces cerevisiae. Mol Cell 16(2):293-9	SGD
detection of sucrose stimulus	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30	Lemaire K, et al.  (2004) Glucose and sucrose act as agonist and mannose as antagonist ligands of the G protein-coupled receptor Gpr1 in the yeast Saccharomyces cerevisiae. Mol Cell 16(2):293-9	SGD
G-protein coupled receptor signaling pathway	IPI: Inferred from Physical Interaction with SGD:GPA2 Assigned on 2007-11-30	Yun CW, et al.  (1997) G-protein coupled receptor from yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun 240(2):287-92	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2001-07-02 IPI: Inferred from Physical Interaction with SGD:GPA2 Assigned on 2007-11-30	Xue Y, et al.  (1998) GPR1 encodes a putative G protein-coupled receptor that associates with the Gpa2p Galpha subunit and functions in a Ras-independent pathway. EMBO J 17(7):1996-2007	SGD
glucose mediated signaling pathway	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30	Yun CW, et al.  (1998) Gpr1p, a putative G-protein coupled receptor, regulates glucose-dependent cellular cAMP level in yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun 252(1):29-33	SGD
	IGI: Inferred from Genetic Interaction with SGD:GPA2, SGD:SCH9 Assigned on 2007-12-03 IMP: Inferred from Mutant Phenotype Assigned on 2007-12-03	Kraakman L, et al.  (1999) A Saccharomyces cerevisiae G-protein coupled receptor, Gpr1, is specifically required for glucose activation of the cAMP pathway during the transition to growth on glucose. Mol Microbiol 32(5):1002-12	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30	Lemaire K, et al.  (2004) Glucose and sucrose act as agonist and mannose as antagonist ligands of the G protein-coupled receptor Gpr1 in the yeast Saccharomyces cerevisiae. Mol Cell 16(2):293-9	SGD
hexose mediated signaling	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30	Lorenz MC, et al.  (2000) The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Genetics 154(2):609-22	SGD
	IGI: Inferred from Genetic Interaction with SGD:GPA2 Assigned on 2007-11-30 IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30	Yun CW, et al.  (1998) Gpr1p, a putative G-protein coupled receptor, regulates glucose-dependent cellular cAMP level in yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun 252(1):29-33	SGD
invasive growth in response to glucose limitation	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-03	Harashima T and Heitman J  (2002) The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits. Mol Cell 10(1):163-73	SGD
pseudohyphal growth	IMP: Inferred from Mutant Phenotype Assigned on 2001-07-02	Lorenz MC, et al.  (2000) The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Genetics 154(2):609-22	SGD
replicative cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-14	Kaeberlein M, et al.  (2005) Genes determining yeast replicative life span in a long-lived genetic background. Mech Ageing Dev 126(4):491-504	SGD
sucrose mediated signaling	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30	Lemaire K, et al.  (2004) Glucose and sucrose act as agonist and mannose as antagonist ligands of the G protein-coupled receptor Gpr1 in the yeast Saccharomyces cerevisiae. Mol Cell 16(2):293-9	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2007-11-30	Yun CW, et al.  (1997) G-protein coupled receptor from yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun 240(2):287-92	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-07-02	Lorenz MC, et al.  (2000) The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Genetics 154(2):609-22	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-07-02	Xue Y, et al.  (1998) GPR1 encodes a putative G protein-coupled receptor that associates with the Gpa2p Galpha subunit and functions in a Ras-independent pathway. EMBO J 17(7):1996-2007	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
G-protein coupled receptor activity	IEA: Inferred from Electronic Annotation with EBI:KW-0297 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
signal transducer activity	IEA: Inferred from Electronic Annotation with EBI:KW-0807 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
G-protein coupled receptor signaling pathway	IEA: Inferred from Electronic Annotation with EBI:KW-0297 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
signal transduction	IEA: Inferred from Electronic Annotation with EBI:KW-0807 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
plasma membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0039 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-1003 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).