This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-08-04    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
SUMO binding	IDA: Inferred from Direct Assay Assigned on 2007-09-21	Xie Y, et al.  (2007) The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation. J Biol Chem 282(47):34176-84	SGD
	IPI: Inferred from Physical Interaction with SGD:SMT3 Assigned on 2008-03-04	Uzunova K, et al.  (2007) Ubiquitin-dependent Proteolytic Control of SUMO Conjugates. J Biol Chem 282(47):34167-75	SGD
contributes_to ubiquitin-protein ligase activity	IDA: Inferred from Direct Assay Assigned on 2007-09-21	Xie Y, et al.  (2007) The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation. J Biol Chem 282(47):34176-84	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-01-02	Uzunova K, et al.  (2007) Ubiquitin-dependent Proteolytic Control of SUMO Conjugates. J Biol Chem 282(47):34167-75	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-10-13	Ii T, et al.  (2007) The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity. Cell Cycle 6(22):2800-9	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein sumoylation	IGI: Inferred from Genetic Interaction with SGD:UBA2, SGD:SMT3, SGD:NFI1, SGD:UBC9, SGD:AOS1 Assigned on 2006-08-02 IMP: Inferred from Mutant Phenotype Assigned on 2006-08-02	Wang Z, et al.  (2006) Genetic Analysis Connects SLX5 and SLX8 to the SUMO Pathway in Saccharomyces cerevisiae. Genetics 172(3):1499-509	SGD
protein ubiquitination	IDA: Inferred from Direct Assay Assigned on 2007-09-21	Xie Y, et al.  (2007) The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation. J Biol Chem 282(47):34176-84	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-01-02	Uzunova K, et al.  (2007) Ubiquitin-dependent Proteolytic Control of SUMO Conjugates. J Biol Chem 282(47):34167-75	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-10-13	Ii T, et al.  (2007) The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity. Cell Cycle 6(22):2800-9	SGD
response to DNA damage stimulus	IGI: Inferred from Genetic Interaction Assigned on 2006-08-04 IMP: Inferred from Mutant Phenotype Assigned on 2006-08-04	Zhang C, et al.  (2006) Suppression of genomic instability by SLX5 and SLX8 in Saccharomyces cerevisiae. DNA Repair (Amst) 5(3):336-46	SGD
	IGI: Inferred from Genetic Interaction with SGD:TOP3, SGD:SGS1 Assigned on 2006-08-04 IMP: Inferred from Mutant Phenotype Assigned on 2006-08-04	Mullen JR, et al.  (2001) Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae. Genetics 157(1):103-18	SGD
telomere maintenance	IMP: Inferred from Mutant Phenotype Assigned on 2006-08-04	Azam M, et al.  (2006) Evidence that the S.cerevisiae Sgs1 protein facilitates recombinational repair of telomeres during senescence. Nucleic Acids Res 34(2):506-16	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2009-03-25	Cook CE, et al.  (2009) The SUMO-targeted ubiquitin ligase subunit Slx5 resides in nuclear foci and at sites of DNA breaks. Cell Cycle 8(7):1080-9	SGD
ubiquitin ligase complex	IDA: Inferred from Direct Assay Assigned on 2007-09-21	Xie Y, et al.  (2007) The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation. J Biol Chem 282(47):34176-84	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SLX5

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ligase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0436 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
zinc ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR001841 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA repair	IEA: Inferred from Electronic Annotation with EBI:KW-0234 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein ubiquitination	IEA: Inferred from Electronic Annotation with UniPathway:UPA00143 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
response to DNA damage stimulus	IEA: Inferred from Electronic Annotation with EBI:KW-0227 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).