This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-12-02    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein serine/threonine phosphatase activity	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Cheng A, et al.  (1999) Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev 13(22):2946-57	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
inactivation of MAPK activity involved in osmosensory signaling pathway	IGI: Inferred from Genetic Interaction Assigned on 2004-10-19 IMP: Inferred from Mutant Phenotype Assigned on 2004-10-19 IDA: Inferred from Direct Assay Assigned on 2004-10-19	Warmka J, et al.  (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21(1):51-60	SGD
mitochondrion inheritance	IMP: Inferred from Mutant Phenotype Assigned on 2002-12-02	Roeder AD, et al.  (1998) Mitochondrial inheritance is delayed in Saccharomyces cerevisiae cells lacking the serine/threonine phosphatase PTC1. Mol Biol Cell 9(4):917-30	SGD
pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP: Inferred from Mutant Phenotype Assigned on 2010-08-12	Malleshaiah MK, et al.  (2010) The scaffold protein Ste5 directly controls a switch-like mating decision in yeast. Nature 465(7294):101-5	SGD
protein dephosphorylation	IDA: Inferred from Direct Assay Assigned on 2002-12-02	Cheng A, et al.  (1999) Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev 13(22):2946-57	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-10-19	Warmka J, et al.  (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21(1):51-60	SGD
tRNA splicing, via endonucleolytic cleavage and ligation	IMP: Inferred from Mutant Phenotype Assigned on 2002-12-02	Robinson MK, et al.  (1994) TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation. Mol Cell Biol 14(6):3634-45	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-12-02	Warmka J, et al.  (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21(1):51-60	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-12-02	Warmka J, et al.  (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21(1):51-60	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for PTC1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR001932 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphoprotein phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0904 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with IUBMB:3.1.3.16 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
protein serine/threonine phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000222 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein dephosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR000222 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
response to stress	IEA: Inferred from Electronic Annotation with EBI:KW-0346 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).