This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-06-25    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2010-06-21	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
proteasomal ubiquitin-dependent protein catabolic process	IDA: Inferred from Direct Assay Assigned on 2010-06-21	Prakash S, et al.  (2009) Substrate selection by the proteasome during degradation of protein complexes. Nat Chem Biol 5(1):29-36	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-06-21	Verma R, et al.  (2001) Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields active S phase cyclin-Cdk. Mol Cell 8(2):439-48	SGD
proteasomal ubiquitin-independent protein catabolic process	IDA: Inferred from Direct Assay Assigned on 2010-06-21	Baugh JM, et al.  (2009) Proteasomes can degrade a significant proportion of cellular proteins independent of ubiquitination. J Mol Biol 386(3):814-27	SGD
proteasome assembly	IGI: Inferred from Genetic Interaction with SGD:BLM10 Assigned on 2008-07-24	Marques AJ, et al.  (2007) The C-terminal Extension of the 7 Subunit and Activator Complexes Stabilize Nascent 20 S Proteasomes and Promote Their Maturation. J Biol Chem 282(48):34869-76	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum membrane	IC: Inferred By Curator from proteasome core complex, beta-subunit complex Assigned on 2010-06-21	Groll M, et al.  (1997) Structure of 20S proteasome from yeast at 2.4 A resolution. Nature 386(6624):463-71	SGD
nucleus	IC: Inferred By Curator from proteasome core complex, beta-subunit complex Assigned on 2010-06-21	Groll M, et al.  (1997) Structure of 20S proteasome from yeast at 2.4 A resolution. Nature 386(6624):463-71	SGD
proteasome core complex, beta-subunit complex	IDA: Inferred from Direct Assay Assigned on 2010-06-21	Groll M, et al.  (1997) Structure of 20S proteasome from yeast at 2.4 A resolution. Nature 386(6624):463-71	SGD
proteasome storage granule	IDA: Inferred from Direct Assay Assigned on 2008-06-25	Laporte D, et al.  (2008) Reversible cytoplasmic localization of the proteasome in quiescent yeast cells. J Cell Biol 181(5):737-45	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for PRE4

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
endopeptidase activity	IEA: Inferred from Electronic Annotation with EBI:IPR016050 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
peptidase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0645 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
threonine-type endopeptidase activity	IEA: Inferred from Electronic Annotation with EBI:IPR016295, EBI:IPR001353, EBI:IPR023333 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0888 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
proteolysis	IEA: Inferred from Electronic Annotation with EBI:KW-0645 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
proteolysis involved in cellular protein catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR016050, EBI:IPR001353 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
proteasome complex	IEA: Inferred from Electronic Annotation with EBI:KW-0647 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
proteasome core complex	IEA: Inferred from Electronic Annotation with EBI:IPR016050, EBI:IPR001353 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).