This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2005-10-20    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IDA: Inferred from Direct Assay Assigned on 2012-03-05	Da G, et al.  (2006) Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes. Proc Natl Acad Sci U S A 103(7):2057-62	SGD
contributes_to DNA translocase activity	IDA: Inferred from Direct Assay Assigned on 2012-04-25	Saha A, et al.  (2002) Chromatin remodeling by RSC involves ATP-dependent DNA translocation. Genes Dev 16(16):2120-34	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP-dependent chromatin remodeling	IDA: Inferred from Direct Assay Assigned on 2004-05-04	Saha A, et al.  (2002) Chromatin remodeling by RSC involves ATP-dependent DNA translocation. Genes Dev 16(16):2120-34	UniProtKB
	IDA: Inferred from Direct Assay Assigned on 2004-05-26	Cairns BR, et al.  (1996) RSC, an essential, abundant chromatin-remodeling complex. Cell 87(7):1249-60	UniProtKB
double-strand break repair via nonhomologous end joining	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-20 IDA: Inferred from Direct Assay Assigned on 2005-10-20	Shim EY, et al.  (2005) The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Mol Cell Biol 25(10):3934-44	SGD
G1/S transition of mitotic cell cycle	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-13	Campsteijn C, et al.  (2007) Reverse genetic analysis of the yeast RSC chromatin remodeler reveals a role for RSC3 and SNF5 homolog 1 in ploidy maintenance. PLoS Genet 3(6):e92	SGD
nucleosome disassembly	IDA: Inferred from Direct Assay Assigned on 2008-09-10	Lorch Y, et al.  (2006) Chromatin remodeling by nucleosome disassembly in vitro. Proc Natl Acad Sci U S A 103(9):3090-3	SGD
regulation of transcription, DNA-dependent	IMP: Inferred from Mutant Phenotype Assigned on 2004-03-26	Moreira JM and Holmberg S  (1999) Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC. EMBO J 18(10):2836-44	UniProtKB
transcription elongation from RNA polymerase II promoter	IDA: Inferred from Direct Assay Assigned on 2007-03-29	Carey M, et al.  (2006) RSC exploits histone acetylation to abrogate the nucleosomal block to RNA polymerase II elongation. Mol Cell 24(3):481-7	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
RSC complex	IDA: Inferred from Direct Assay Assigned on 2007-01-19	Cairns BR, et al.  (1996) RSC, an essential, abundant chromatin-remodeling complex. Cell 87(7):1249-60	UniProtKB
	IDA: Inferred from Direct Assay Assigned on 2007-01-23	Saha A, et al.  (2002) Chromatin remodeling by RSC involves ATP-dependent DNA translocation. Genes Dev 16(16):2120-34	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-01-31	Cairns BR, et al.  (1999) Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains. Mol Cell 4(5):715-23	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin binding	IEA: Inferred from Electronic Annotation with EBI:IPR001005 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR009057 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
zinc ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR000433 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin modification	IEA: Inferred from Electronic Annotation with EBI:KW-0156 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).